PMID: 8602349Feb 1, 1996Paper

Nucleosome positioning properties of the albumin transcriptional enhancer

Nucleic Acids Research
C E McPhersonK S Zaret

Abstract

Considering the importance of nucleosome position with regard to how regulatory factors recognize their binding sites in chromatin, we have investigated the inherent nucleosome positioning properties of a transcriptional enhancer of the albumin gene. In the liver, where the albumin gene is highly expressed, the enhancer exists in an array of precisely positioned, nucleosome-like particles with transcription factors bound. In the absence of specific binding factors, such as in non-liver tissues or in polynucleosome arrays assembled in vitro, nucleosomes are randomly positioned over the enhancer. Herein we investigate the intrinsic nucleosome positioning properties of the central enhancer sequence assembled into mononucleosome core particles in vitro. We find that the enhancer DNA prefers three translational positions, each of which utilizes different rotational settings on the nucleosome core. We conclude that DNA binding factors that position nucleosomes may do so by stabilizing one configuration out of several that can be adopted by the underlying DNA, and that the potential exists for different positions to be stabilized at different stages of development.

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Citations

Sep 26, 2002·Developmental Biology·Peter Carlsson, Margit Mahlapuu
Feb 1, 1997·Current Opinion in Structural Biology·S K BurleyF Shu
Mar 12, 2002·Insect Biochemistry and Molecular Biology·E JulienP Couble
Feb 21, 1998·Genes & Development·E Y ShimK S Zaret
May 23, 2006·Clinical Biochemistry·Theodore PetersMonica Galliano
Jun 8, 2000·The Journal of Biological Chemistry·W R VorachekJ Locker
Oct 10, 2007·The Journal of Biological Chemistry·Mitsutoki Hatta, Lisa Ann Cirillo
Aug 5, 1998·The American Journal of Physiology·H OkadaE G Neilson
Nov 10, 2009·The Journal of Biological Chemistry·Sarah Kohler, Lisa Ann Cirillo
Jul 19, 2002·FEBS Letters·Ryoiti Kiyama, Edward N Trifonov
Mar 23, 2018·Open Biology·Yoshimasa TakizawaHitoshi Kurumizaka
Sep 22, 2001·Current Biology : CB·A K BielinskyS A Gerbi
Dec 17, 2014·Genes & Development·Makiko Iwafuchi-Doi, Kenneth S Zaret

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