Nucleosome sliding induced by the xMi-2 complex does not occur exclusively via a simple twist-diffusion mechanism.

The Journal of Biological Chemistry
Sayura AoyagiJeffrey J Hayes

Abstract

ATP-dependent chromatin remodeling complexes can induce the translocation (sliding) of nucleosomes in cis along DNA, but the mechanism by which sliding occurs is not well defined. We previously presented evidence that sliding induced by the human SWI/SNF complex does not occur solely via a proposed "twist-diffusion" mechanism whereby the DNA rotates about its helical axis without displacement from the surface of the nucleosome (Aoyagi, S., and Hayes, J. J. (2002) Mol. Cell. Biol. 22, 7484-7490). Here we examined whether the Xenopus Mi-2 nucleosome remodeling complex induces nucleosome sliding via a twist-diffusion mechanism with nucleosomes assembled onto DNA templates containing branched DNA structures expected to sterically hinder rotation of the DNA helix on the nucleosome surface. We find that the branched DNA-containing nucleosomes undergo xMi-2-catalyzed sliding at a rate and extent identical to that of nucleosomes assembled on native DNA fragments. These results indicate that both the hSWI/SNF and xMi-2 complexes induce nucleosome sliding via a mechanism(s) other than simple twist diffusion and are consistent with models in which the DNA largely maintains its rotational orientation with respect to the histone surface.

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Citations

Jun 11, 2008·Cell Biochemistry and Biophysics·Gavin R Schnitzler
Mar 17, 2004·Biochimica Et Biophysica Acta·Gernot Längst, Peter B Becker
Mar 9, 2004·Trends in Biochemical Sciences·Vasily M StuditskyGary Felsenfeld
Sep 7, 2013·Nature Structural & Molecular Biology·Felix Mueller-PlanitzPeter B Becker
Sep 6, 2005·Nature Structural & Molecular Biology·Peter B Becker
Apr 2, 2011·The Journal of Biological Chemistry·Indu JagannathanJeffrey J Hayes
Jun 16, 2004·Current Opinion in Genetics & Development·Andrew Flaus, Tom Owen-Hughes
May 1, 2007·Biophysical Journal·Fabien MontelCendrine Faivre-Moskalenko
Jun 16, 2015·Genes·Gernot Längst, Laura Manelyte
Jan 20, 2007·The Journal of Biological Chemistry·Zungyoon YangJeffrey J Hayes
Jan 19, 2011·Chembiochem : a European Journal of Chemical Biology·Ning LiuJeffrey J Hayes
Jan 5, 2021·Annual Review of Biophysics·Ilana M Nodelman, Gregory D Bowman

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