Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density

Molecular and Cellular Biology
Corinna LielegPhilipp Korber

Abstract

Arrays of regularly spaced nucleosomes are a hallmark of chromatin, but it remains unclear how they are generated. Recent genome-wide studies, in vitro and in vivo, showed constant nucleosome spacing even if the histone concentration was experimentally reduced. This counters the long-held assumption that nucleosome density determines spacing and calls for factors keeping spacing constant regardless of nucleosome density. We call this a clamping activity. Here, we show in a purified system that ISWI- and CHD1-type nucleosome remodelers have a clamping activity such that they not only generate regularly spaced nucleosome arrays but also generate constant spacing regardless of nucleosome density. This points to a functionally attractive nucleosome interaction that could be mediated either directly by nucleosome-nucleosome contacts or indirectly through the remodelers. Mutant Drosophila melanogaster ISWI without the Hand-Sant-Slide (HSS) domain had no detectable spacing activity even though it is known to remodel and slide nucleosomes. This suggests that the role of ISWI remodelers in generating constant spacing is not just to mediate nucleosome sliding; they actively contribute to the attractive interaction. Additional factors are...Continue Reading

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Citations

Feb 11, 2016·Nucleic Acids Research·Josefina OcampoDavid J Clark
Jul 9, 2016·Annual Review of Biophysics·Coral Y ZhouGeeta J Narlikar
Aug 30, 2016·Cell Reports·Pauline VasseurMarta Radman-Livaja
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Jan 22, 2017·ELife·Johanna LudwigsenFelix Mueller-Planitz
Oct 12, 2017·Nature·Lucas FarnungPatrick Cramer
Nov 7, 2019·Genome Research·Elisa OberbeckmannPhilipp Korber
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May 30, 2021·Nature Communications·Elisa OberbeckmannPhilipp Korber
May 30, 2021·Nature Communications·Elisa OberbeckmannSebastian Eustermann
Jul 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xinya HuangXuezhu Feng
Dec 24, 2020·Genome Research·Etienne RouthierJulien Mozziconacci
Dec 3, 2021·Nature Communications·Ashish Kumar SinghFelix Mueller-Planitz

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