Nucleosome structural transition during chromatin unfolding is caused by conformational changes in nucleosomal DNA.

The Journal of Biological Chemistry
I M GavinS G Bavykin

Abstract

We have recently reported that certain core histone-DNA contacts are altered in nucleosomes during chromatin unfolding (Usachenko, S. I., Gavin I. M., and Bavykin, S. G. (1996) J. Biol. Chem. 271, 3831-3836). In this work, we demonstrate that these alterations are caused by a conformational change in the nucleosomal DNA. Using zero-length protein-DNA cross-linking, we have mapped histone-DNA contacts in isolated core particles at ionic conditions affecting DNA stiffness, which may change the nucleosomal DNA conformation. We found that the alterations in histone-DNA contacts induced by an increase in DNA stiffness in isolated core particles are identical to those observed in nucleosomes during chromatin unfolding. The change in the pattern of micrococcal nuclease digestion of linker histone-depleted chromatin at ionic conditions affecting chromatin compaction also suggests that the stretching of the linker DNA may alter the nucleosomal DNA conformation, resulting in a structural transition in the nucleosome which may play a role in rendering the nucleosome competent for transcription and/or replication.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A Ruiz-CarrilloR Lurz
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D Lohr, K E Van Holde
May 25, 1979·Science·A PrunellF H Crick
Sep 4, 1979·Biochemistry·H M WuD M Crothers
Jan 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Gruss, J M Sogo
Mar 5, 1992·Journal of Molecular Biology·M M StruckT J Richmond
Jan 1, 1992·Trends in Biochemical Sciences·R H Morse
Dec 2, 1991·Biochimica Et Biophysica Acta·N Davies, G G Lindsey
Aug 27, 1991·Biochemistry·J YaoJ Widom
Sep 1, 1990·Molecular and Cellular Biology·E C Park, J W Szostak
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L M JohnsonM Grunstein
Feb 20, 1990·Journal of Molecular Biology·D J Clark, T Kimura
Apr 5, 1990·Journal of Molecular Biology·S G BavykinA D Mirzabekov
Aug 6, 1987·Nature·M E HoganR H Austin
Apr 1, 1989·Journal of Biomolecular Structure & Dynamics·G S ManningA Rich
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·J Widom
Aug 1, 1989·Journal of Biomolecular Structure & Dynamics·E C Uberbacher, G J Bunick
Jan 1, 1989·Methods in Enzymology·A D MirzabekovK K Ebralidse
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·P J Hagerman
Mar 20, 1988·Journal of Molecular Biology·I GouletB Revet
Jan 1, 1986·CRC Critical Reviews in Biochemistry·R S WuW M Bonner
Jul 6, 1982·Biochemistry·L J Libertini, E W Small
May 25, 1980·Journal of Molecular Biology·V V ShickA D Mirzabekov
May 25, 1980·Journal of Molecular Biology·A V BelyavskyA D Mirzabekov
Jul 15, 1995·Genes & Development·P C MegeeM M Smith
Jul 1, 1994·Current Biology : CB·A A Travers
Jan 1, 1993·Cold Spring Harbor Symposia on Quantitative Biology·T J RichmondK Luger

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Citations

Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·D Kültz, D Chakravarty
Jan 16, 1999·Nucleic Acids Research·A P Wolffe, J J Hayes
Oct 27, 2004·Biochemical and Biophysical Research Communications·Mogens Engelhardt
Sep 10, 1999·The Journal of Biological Chemistry·D J Fitzgerald, J N Anderson

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