Nucleosomes are translationally positioned on the active allele and rotationally positioned on the inactive allele of the HPRT promoter

Molecular and Cellular Biology
C Chen, T Yang

Abstract

Differential chromatin structure is one of the hallmarks distinguishing active and inactive genes. For the X-linked human hypoxanthine phosphoribosyltransferase gene (HPRT), this difference in chromatin structure is evident in the differential general DNase I sensitivity and hypersensitivity of the promoter regions on active versus inactive X chromosomes. Here we characterize the nucleosomal organization responsible for the differential chromatin structure of the active and inactive HPRT promoters. The micrococcal nuclease digestion pattern of chromatin from the active allele in permeabilized cells reveals an ordered array of translationally positioned nucleosomes in the promoter region except over a 350-bp region that is either nucleosome free or contains structurally altered nucleosomes. This 350-bp region includes the entire minimal promoter and all of the multiple transcription initiation sites of the HPRT gene. It also encompasses all of the transcription factor binding sites identified by either dimethyl sulfate or DNase I in vivo footprinting of the active allele. In contrast, analysis of the inactive HPRT promoter reveals no hypersensitivity to either DNase I or a micrococcal nuclease and no translational positioning of...Continue Reading

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Citations

Dec 5, 2002·Chembiochem : a European Journal of Chemical Biology·Micaela CasertaErnesto Di Mauro
Jan 16, 2007·Nature Biotechnology·Fatih OzsolakDavid E Fisher
Nov 11, 2009·The Journal of Biological Chemistry·Mohamed El GazzarCharles E McCall
Feb 21, 2004·Nucleic Acids Research·Li ChenErik Bateman
Aug 24, 2005·Nucleic Acids Research·Sara Rodriguez-JatoThomas P Yang
May 3, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·François Vigneault, Régen Drouin
Oct 1, 2005·BMC Genetics·Catherine L Anderson, Carolyn J Brown
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Jun 1, 2014·Nucleic Acids Research·Shinichi Kadota, Kyosuke Nagata
Nov 28, 2012·International Journal for Parasitology·Yingling HuangDavid R Allred
Dec 12, 2002·The Journal of Biological Chemistry·Corey D BraastadEric A Hendrickson
Jun 5, 2021·Journal of Cellular and Molecular Medicine·Alexei G Basnakian, Christopher L Moore
Jan 26, 2005·Journal of Cellular Biochemistry·Meredith L HanelRachel Wevrick

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