PMID: 7335494Dec 21, 1981Paper

Nucleosomes will not form on double-stranded RNa or over poly(dA).poly(dT) tracts in recombinant DNA

Nucleic Acids Research
G R Kunkel, H G Martinson

Abstract

We have been unable to "force" double-stranded RNA to fold into nucleosome-like structures using several different histone-RNA "reconstitution" procedures. Even if the histones are first stabilized in octameric form by dimethylsuberimidate cross-linking they are still unable to form specific complexes with the RNA. Moreover double-stranded RNA is unable to induce histones to assemble into octamers although we confirm that the non-nucleic acid homopolymer, polyglutamic acid, has this ability. We have also determined, using pyrimidine tract analysis, that nucleosomes will not form over a sufficiently long segment of poly(dA).poly(dT) in a recombinant DNA molecule. Thus nucleosomes cannot fold DNA containing an 80 base pair poly(dA).poly(dT) segment but a 20 base pair segment can be accommodated in nucleosomes fairly well. Segments of intermediate length can be accommodated but are clearly selected against. Poly(dA).poly(dT) differs only slightly from natural DNA in helix structure. Therefore either this homopolymer resists folding, or nucleosomes are very exacting in the nucleic acid steroid parameters they will tolerate. Such constraints may be relevant to nucleosome positioning in chromatin.

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A SteinR T Simpson
Oct 1, 1978·Nucleic Acids Research·P N BryanD E Olins
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·H C BirnboimV L Seligy
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H G MartinsonG Kunkel
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A D Mirzabekov, A Rich
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A SteinM Bina
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M PhilipH S Chandra
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R A FirtelM McKeown
Jun 16, 1977·Nature·A M MaxamW Gilbert
May 1, 1977·Quarterly Reviews of Biophysics·N R Kallenbach, H M Berman
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H M Fried, G R Fink
Nov 10, 1979·Nucleic Acids Research·R I RichardsH M Goodman
Nov 29, 1977·Biochemistry·K Tatchell, K E Van Holde
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H C BirnboimN A Straus
Feb 11, 1980·Nucleic Acids Research·K Dunn, J D Griffith
Jul 23, 1981·Nature·L J Peck, J C Wang
Jul 23, 1981·Nature·D Rhodes, A Klug
Jun 25, 1981·Nucleic Acids Research·C DingwallR A Laskey
Jul 15, 1981·Journal of Molecular Biology·R E Dickerson, H R Drew
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Grosschedl, M L Birnstiel
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S B Zimmerman, B H Pheiffer
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G VogeliB de Crombrugghe
Jan 1, 1980·Annual Review of Biochemistry·J D McGhee, G Felsenfeld
Jan 1, 1981·Analytical Biochemistry·C K Lau, J M Fujitaki
Jan 11, 1980·Nucleic Acids Research·M NollJ Dubochet
Oct 23, 1980·Nature·R WingR E Dickerson
Oct 15, 1980·Journal of Molecular Biology·A G LeslieR L Ratliff
Sep 11, 1980·Nucleic Acids Research·E N Trifonov

❮ Previous
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Citations

Jul 1, 1987·Plant Molecular Biology·R J FerlB H Laughner
Mar 1, 1988·Molecular & General Genetics : MGG·S Y KimS M Kingsman
May 1, 1983·Molecular Biology Reports·R Tsanev
Sep 1, 1995·Journal of Molecular Evolution·T A EppC C Liew
Jun 1, 1989·Molecular & General Genetics : MGG·J F Martínez-GarcíaJ E Pérez-Ortín
Feb 26, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yong-qiang XingLu Cai
Sep 1, 1995·Insect Biochemistry and Molecular Biology·M YussaE Abdelhay
Jun 11, 2009·Quarterly Reviews of Biophysics·Tali E Haran, Udayan Mohanty
Jul 5, 2006·Nature Structural & Molecular Biology·Iestyn Whitehouse, Toshio Tsukiyama
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C ThrashR Sternglanz
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K Struhl
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K R Hovatter, H G Martinson
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R E DickersonS Neidle
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y H Wang, J D Griffith
Feb 2, 2012·Journal of Biomolecular Structure & Dynamics·Zhiqian ZhangIvan Gutman
Aug 1, 1985·Journal of Biomolecular Structure & Dynamics·D M Soumpasis
Dec 1, 1988·Journal of Biomolecular Structure & Dynamics·R NussinovR L Jernigan
Feb 1, 1990·Journal of Biomolecular Structure & Dynamics·A A Lipanov Skuratovskii IYa
Aug 1, 1992·Journal of Biomolecular Structure & Dynamics·R Chandrasekaran, A Radha
Oct 1, 1994·Journal of Biomolecular Structure & Dynamics·H StaffelbachC Burks
Sep 19, 2003·Molecular Biology of the Cell·Christopher W EspelinPeter K Sorger
Jul 24, 1982·Nucleic Acids Research·R HeiligJ L Mandel
Jun 25, 1983·Nucleic Acids Research·S ArnottL C Puigjaner
Nov 11, 1986·Nucleic Acids Research·J N Anderson
Mar 11, 1986·Nucleic Acids Research·R E EllisA R Coulson
Aug 11, 1987·Nucleic Acids Research·S Diekmann, D A Zarling
Feb 25, 1988·Nucleic Acids Research·A M Beasty, M J Behe
Mar 25, 1990·Nucleic Acids Research·S Brahms, J G Brahms

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