Nucleotide-binding oligomerization domain 2 (NOD2) activation induces apoptosis of human oral squamous cell carcinoma cells

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Hyo-Eun YoonJung-Hoon Yoon

Abstract

Microbial Pattern-recognition receptors (PRRs), such as nucleotide-binding oligomerization domains (NODs), are essential for mammalian innate immune response. This study was designed to determine the effect of NOD1 and NOD2 agonist on innate immune responses and antitumor activity in oral squamous cell carcinoma (OSCC) cells. NODs expression was examined by RT-PCR, and IL-8 production by NODs agonist was examined by ELISA. Western blot analysis was performed to determine the MAPK activation in response to their agonist. Cell proliferation was determined by MTT assay. Flow cytometry and Western blot analysis were performed to determine the MDP-induced cell death. The levels of NODs were apparently expressed in OSCC cells. NODs agonist, Tri-DAP and MDP, led to the production of IL-8 and MAPK activation. NOD2 agonist, MDP, inhibited the proliferation of YD-10B cells in a dose-dependent manner. Also, the ratio of Annexin V-positive cells and cleaved PARP was increased by MDP treatment in YD-10B cells, suggesting that MDP-induced cell death in YD-10B cells may be owing to apoptosis. Our results indicate that NODs are functionally expressed in OSCC cells and can trigger innate immune responses. In addition, NOD2 agonist inhibited cel...Continue Reading

References

Jul 21, 2001·EMBO Reports·S E GirardinD J Philpott
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gerard J NauRichard A Young
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Jul 23, 2003·The Journal of Biological Chemistry·Stephen E GirardinDominique Mengin-Lecreulx
Oct 16, 2003·Molecular and Cellular Biology·Anne-Laure Pauleau, Peter J Murray
Apr 20, 2004·Cancer Cell·Li MaoVassiliki A Papadimitrakopoulou
Jun 9, 2004·International Journal of Cancer. Journal International Du Cancer·Colin HopperGraham Putnam
Aug 27, 2004·Nature·Eli PikarskyYinon Ben-Neriah
Oct 19, 2004·Nature Immunology·Jérôme VialaRichard L Ferrero
Sep 22, 2005·Nature Reviews. Immunology·Michael Karin, Florian R Greten
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean da Silva CorreiaRichard J Ulevitch
May 26, 2006·Journal of Dental Research·Y SugawaraH Takada
Jul 13, 2006·Expert Review of Anticancer Therapy·David ChinWilliam B Coman
Sep 12, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Martin J RicherMarc S Horwitz
Feb 13, 2007·Current Opinion in Immunology·Jack D Bui, Robert D Schreiber
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jong-Hwan ParkGabriel Núñez
May 31, 2008·Contributions to Microbiology·Thomas Areschoug, Siamon Gordon
Jul 9, 2008·Biochemistry. Biokhimii︠a︡·S Rakoff-Nahoum, R Medzhitov
Feb 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alana A ShigeokaDianne B McKay
May 12, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jong-Hwan ParkJung-Hoon Yoon
Nov 18, 2010·Human Immunology·Anton G Kutikhin
Jul 13, 2011·Human Immunology·Anton G Kutikhin
Dec 4, 2013·Inflammatory Bowel Diseases·Alice Y ChuangJohn H Kwon
Dec 20, 2014·Immunity·Roberta CarusoGabriel Núñez

❮ Previous
Next ❯

Citations

Oct 28, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Jiajie LiShengfu Huang
Aug 5, 2021·Signal Transduction and Targeted Therapy·Danyang Li, Minghua Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis