PMID: 11342042May 9, 2001Paper

Nucleotide binding to the chaperonin GroEL: non-cooperative binding of ATP analogs and ADP, and cooperative effect of ATP

Biochimica Et Biophysica Acta
T InobeK Kuwajima

Abstract

Chaperonin-assisted protein folding proceeds through cycles of ATP binding and hydrolysis by GroEL, which undergoes a large structural change by the ATP binding or hydrolysis. One of the main concerns of GroEL is the mechanism of the productive and cooperative structural change of GroEL induced by the nucleotide. We studied the cooperative nature of GroEL by nucleotide titration using isothermal titration calorimetry and fluorescence spectroscopy. Our results indicated that the binding of ADP and ATP analogs to a single ring mutant (SR1), as well as that to GroEL, was non-cooperative. Only ATP induces an apparently cooperative conformational change in both proteins. Furthermore, the fluorescence changes of pyrene-labeled GroEL indicated that GroEL has two kinds of nucleotide binding sites. The fluorescence titration result fits well with a model in which two kinds of binding sites are both non-cooperative and independent of each other. These results suggest that the binding and hydrolysis of ATP may be necessary for the cooperative transition of GroEL.

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Citations

May 14, 2003·Journal of Molecular Biology·Tomonao InobeKunihiro Kuwajima
Dec 14, 2002·Biochimica Et Biophysica Acta·Nadja Hellmann, Heinz Decker
Jun 1, 2002·Journal of Biological Physics·K KuwajimaT Inobe
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gabriel A FrankGilad Haran
Jul 13, 2012·Journal of Virology·Lidia P KurochkinaVadim V Mesyanzhinov
Feb 25, 2014·Journal of Biochemistry·So IshinoMasaru Hoshino
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oded DanzigerAmnon Horovitz
Jul 5, 2015·FEBS Letters·Lars SkjærvenJosé María Valpuesta
Jan 5, 2016·Chemical Reviews·Ranit Gruber, Amnon Horovitz
Jul 1, 2004·Molecular Microbiology·Manuel FerrerPeter N Golyshin
Jun 19, 2008·Protein Science : a Publication of the Protein Society·Qiang Cui, Martin Karplus
May 5, 2006·Protein Science : a Publication of the Protein Society·Oded DanzigerAmnon Horovitz
May 5, 2004·Journal of Molecular Biology·Tomonao Inobe, Kunihiro Kuwajima
Jul 9, 2004·Biophysical Journal·Arjan van der VaartMartin Karplus
Oct 3, 2001·Journal of Structural Biology·A HorovitzO Yifrach
Nov 10, 2013·PloS One·Toshifumi MizutaTomohiro Mizobata
Oct 24, 2017·PloS One·Toshio TakenakaKunihiro Kuwajima
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May 11, 2006·Chemical Reviews·Arthur L HorwichWayne A Fenton
Jan 5, 2010·Biochimica Et Biophysica Acta·Shinji TanakaMasaru Hoshino

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