Nucleotide exchange on ARF mediated by yeast Gea1 protein

Nature
A PeyrocheC L Jackson

Abstract

The ADP-ribosylation factor ARF is a small GTP-binding protein that is involved in the transport of vesicles between the endoplasmic reticulum (ER) and Golgi complex and within the Golgi complex itself. ARF cycles between inactive and membrane-associated active forms as a result of exchange of bound GDP for GTP; the GTP-bound form is an essential participant in the formation of transport vesicles. This nucleotide exchange is inhibited by the fungal metabolite brefeldin A (BFA). Here we identify a protein (Gea1) from Saccharomyces cerevisiae that is a component of a complex possessing guanine-nucleotide-exchange activity for ARF. We show that the activity of the complex is sensitive to brefeldin A and that Gea1 function is necessary for ER-Golgi transport in vivo. Gea1 contains a domain that is similar to a domain of Sec7, a protein necessary for intra-Golgi transport. We propose that Gea1 and ARNO, a human protein with a homologous Sec7 domain, are members of a new family of ARF guanine-nucleotide exchange factors.

References

Feb 1, 1992·Yeast·D MuhlradR Parker
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J G DonaldsonR D Klausner
Apr 1, 1995·Trends in Biochemical Sciences·A L Boman, R A Kahn
Nov 3, 1994·Nature·J E Rothman
Dec 30, 1994·Cell·H R Pelham
Nov 1, 1993·Molecular & General Genetics : MGG·K FujimuraA Toh-e
Mar 15, 1996·Science·R Schekman, L Orci
Apr 12, 1996·Science·J E Rothman, F T Wieland
Jul 1, 1996·Molecular and Cellular Biology·S B DeitzA Franzusoff

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Citations

Oct 3, 1999·Cell Motility and the Cytoskeleton·A L BomanR A Kahn
Sep 9, 2000·Biochimica Et Biophysica Acta·N GodaG Tsujimoto
Aug 26, 1998·Biochimica Et Biophysica Acta·J Hirst, M S Robinson
Oct 18, 2002·Current Opinion in Cell Biology·Anne Spang
Aug 1, 1997·Current Opinion in Cell Biology·M G Roth, P C Sternweis
Aug 1, 1997·Current Opinion in Cell Biology·L M Traub, S Kornfeld
Jan 13, 1998·Current Opinion in Cell Biology·G JürgensT Steinmann
Feb 1, 2000·Trends in Cell Biology·C L Jackson, J E Casanova
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pei-Chin TsaiFang-Jen S Lee
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·N MorinagaM Vaughan
Jul 23, 2002·Molecular Biology of the Cell·Cecilia J BonangelinoJuan S Bonifacino
May 15, 2002·Molecular Biology of the Cell·Eleanor S ClickDavid Botstein
Aug 6, 1999·Molecular Biology of the Cell·J HirstM S Robinson
Feb 13, 2001·Molecular Biology of the Cell·N YaharaA Nakano
Apr 4, 1998·Molecular Biology of the Cell·E C GaynorT R Graham
Jun 17, 2003·Molecular Biology of the Cell·Sophie ChantalatAnne Peyroche
Sep 16, 2003·Molecular Biology of the Cell·Chun-Fang HuangFang-Jen S Lee
Jan 27, 2004·Molecular Biology of the Cell·Randal CoxNava Segev
Jul 16, 2004·Molecular Biology of the Cell·Stephen M LewisAnne Spang
Sep 10, 2004·Molecular Biology of the Cell·Mark TrautweinAnne Spang
Apr 13, 2011·Cold Spring Harbor Perspectives in Biology·Andrés Lorente-Rodríguez, Charles Barlowe
Nov 23, 2000·The Plant Cell·D E ShevellN H Chua
Nov 23, 2000·The Plant Cell·P PimplD G Robinson
Dec 12, 2001·Molecular and Cellular Biology·Hélène Dumay-OdelotChristian Marck
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ben Scheres, Philip N. Benfey
Jan 1, 1997·Annual Review of Cell and Developmental Biology·J E Fowler, R S Quatrano
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Marcus C S LeeRandy Schekman

❮ Previous
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