Nucleotide excision repair capacity increases during differentiation of human embryonic carcinoma cells into neurons and muscle cells.

The Journal of Biological Chemistry
Wentao LiAziz Sancar

Abstract

Embryonic stem cells can self-renew and differentiate, holding great promise for regenerative medicine. They also employ multiple mechanisms to preserve the integrity of their genomes. Nucleotide excision repair, a versatile repair mechanism, removes bulky DNA adducts from the genome. However, the dynamics of the capacity of nucleotide excision repair during stem cell differentiation remain unclear. Here, using immunoslot blot assay, we measured repair rates of UV-induced DNA damage during differentiation of human embryonic carcinoma (NTERA-2) cells into neurons and muscle cells. Our results revealed that the capacity of nucleotide excision repair increases as cell differentiation progresses. We also found that inhibition of the apoptotic signaling pathway has no effect on nucleotide excision repair capacity. Furthermore, RNA-Seq-based transcriptomic analysis indicated that expression levels of four core repair factors, xeroderma pigmentosum (XP) complementation group A (XPA), XPC, XPG, and XPF-ERCC1, are progressively up-regulated during differentiation, but not those of replication protein A (RPA) and transcription factor IIH (TFIIH). Together, our findings reveal that increase of nucleotide excision repair capacity accompani...Continue Reading

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Citations

Oct 31, 2019·Open Biology·Sofia J Araújo, Isao Kuraoka
Sep 25, 2020·Chemical Society Reviews·Cécile MingardShana J Sturla
Oct 29, 2020·Functional & Integrative Genomics·Handan Emisoglu-KulahliNuri Ozturk
Mar 28, 2021·The Journal of Biological Chemistry·Yuchao JiangAziz Sancar

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