Nucleotide-mediated mucin secretion from differentiated human bronchial epithelial cells

American Journal of Respiratory Cell and Molecular Biology
Philip A KempAlan D Jackson

Abstract

Most current cell-based models for examining the regulation of mucin secretion demonstrate low signal-to-noise ratios, making experimental manipulation and data interpretation difficult. Using adenosine triphosphate (ATP) as a mucin secretagogue, we have developed a model of agonist-induced mucin secretion in differentiated human bronchial epithelial cells. Mucin secretory signals were estimated using enzyme-linked lectin assay, and typical signals of 300-400% of baseline were observed in response to a 30-min exposure to ATP (100 microM). ATP and uridine triphosphate equipotently stimulated mucin secretion consistent with mediation via P2Y2 receptor activation. Suramin and AR-C118925XX, a competitive P2Y2 receptor antagonist, inhibited adenosine 5'-o-(3-thiotriphosphate) (ATP-gammaS)-induced mucin secretion. A selective Gq G-protein antagonist (GP-ANT)-2A completely abrogated ATP-gammaS-induced mucin secretion. Pertussis toxin and the G(i/o)-specific, GP-ANT-2, had no effect. The phospholipase C inhibitor, D609, and the protein kinase C inhibitor, calphostin C, substantially inhibited ATP-gammaS-induced mucin secretion. Phorbol myristate acetate also stimulated mucin secretion in a calphostin C-sensitive manner. ATP-gammaS-indu...Continue Reading

References

Dec 1, 1991·Circulation Research·V Ralevic, G Burnstock
May 1, 1991·British Journal of Pharmacology·K C Kim, B C Lee
Oct 1, 1990·Biology of Reproduction·G A BalaJ E Bleasdale
Nov 1, 1990·American Journal of Respiratory Cell and Molecular Biology·R WuR Boucher
Aug 1, 1989·The American Review of Respiratory Disease·T AikawaT Takahashi
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M J StuttsR C Boucher
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R J SmithJ E Bleasdale
Feb 1, 1993·American Journal of Respiratory Cell and Molecular Biology·K C KimI Van-Seuningen
Jan 1, 1996·American Journal of Respiratory Cell and Molecular Biology·T E GrayP Nettesheim
Feb 1, 1996·American Journal of Respiratory Cell and Molecular Biology·S H RandellP Nettesheim
Feb 1, 1997·American Journal of Respiratory Cell and Molecular Biology·K H KoK C Kim
May 1, 1997·Pflügers Archiv : European journal of physiology·J LeipzigerR Greger
Jul 1, 1997·The American Journal of Physiology·L H AbdullahC W Davis
Nov 14, 1997·The Journal of Physiology·Z J Cui, T Kanno
Aug 5, 1998·The American Journal of Physiology·C E ScottC W Davis
Oct 14, 1998·American Journal of Respiratory and Critical Care Medicine·C AgustiJ A Nadel
Oct 21, 1998·Experimental Eye Research·J E Jumblatt, M M Jumblatt
Aug 12, 1999·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·K C KimM K Lee
Jul 27, 2000·American Journal of Physiology. Cell Physiology·H SauerM Wartenberg
Feb 13, 2001·Trends in Pharmacological Sciences·A D Jackson
Mar 10, 2001·Respiration Physiology·D F Rogers
Nov 6, 2001·American Journal of Respiratory Cell and Molecular Biology·Y ChenR Wu
Apr 19, 2002·Journal of the American Society of Nephrology : JASN·Ruisheng LiuA Erik G Persson
Feb 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lubna H AbdullahC William Davis
Jun 26, 2004·The Journal of Physiology·Andrea H RossiC William Davis
Aug 31, 2007·American Journal of Physiology. Cell Physiology·Camille EhreC William Davis

❮ Previous
Next ❯

Citations

Feb 12, 2009·The Journal of Biological Chemistry·Michael J TuvimBurton F Dickey
Dec 17, 2008·Current Opinion in Pulmonary Medicine·Christopher M EvansBurton F Dickey
Nov 9, 2007·Annual Review of Physiology·C William Davis, Burton F Dickey
Feb 2, 2011·PloS One·Eric Y T ChenWei-Chun Chin
Oct 16, 2012·Respiratory Physiology & Neurobiology·Chunyi LiuJuliy M Perelman
Dec 22, 2015·Neuropharmacology·Kenneth A Jacobson, Christa E Müller
Jun 25, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K RöschmannA Petersen
Apr 19, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·E F DiezmosL Liu
Mar 2, 2006·Journal of Pharmacological and Toxicological Methods·Jonathan E PhillipsMichael Minnicozzi
Jul 15, 2016·Journal of Medicinal Chemistry·Sean ConroyMichael J Stocks
Sep 30, 2016·Respiratory Research·Yutaka ShishikuraMasakazu Ichinose
Dec 27, 2005·Respiratory Physiology & Neurobiology·Daisuke InoueHidetada Sasaki
Oct 26, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Veronika E WinkelmannManfred Frick
Mar 7, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·A K M Shamsuddin, Paul M Quinton
Oct 18, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ryota HokariYoung S Kim
Sep 5, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andrea H RossiC William Davis
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
Aug 31, 2007·American Journal of Physiology. Cell Physiology·Camille EhreC William Davis
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Aug 14, 2012·Pharmacological Reviews·Geoffrey BurnstockJean-Pierre Timmermans
Mar 2, 2006·Pharmacological Reviews·Geoffrey Burnstock
Apr 4, 2019·American Journal of Respiratory Cell and Molecular Biology·Aurelie PetitArnaud Bourdin
Oct 18, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Henry DanahayRobert J Bridges
Jan 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·James L KreindlerHenry Danahay
Nov 23, 2019·Respiratory Research·Kalina R Atanasova, Leah R Reznikov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.