PMID: 8939156Nov 1, 1996Paper

Nucleotide sequence analysis of a complementary DNA coding for a Blomia tropicalis allergen

The Journal of Allergy and Clinical Immunology
L PuertaM L Dao

Abstract

Blomia tropicalis is a mite of allergenic importance in tropical and subtropical areas. A clone (Bt11a) from a B. tropicalis complementary DNA library was expressed in lambda phage and analyzed by plaque radioimmunoassay. The recombinant allergen produced by this clone was bound by IgE in 16 of 32 sera from individuals with asthma with a positive RAST response and none of 3 control sera from healthy individuals with negative RAST response to B. tropicalis. The cDNA insert was amplified by polymerase chain reaction with use of universal primers. A 582-base-pair (bp) fragment was cloned into a pCR II vector. The complete sequence of both strands was determined by using T7, SP6, and internal primers. The sequence shows a 432 bp reading frame with a 34 bp 5' untranslated region and a 116 bp 3' untranslated region with a poly A tail. Analysis of the sequence suggests that it encodes a putative signal peptide of 20 residues and a 124-residue mature protein allergen of 14,206 Da. The nucleotide and the inferred amino acid sequences did not show homology to any known sequence. No potential N-linked glycosylation site was found. The recombinant protein appears to represent a major allergen of the mite B. tropicalis.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K Y ChuaW R Thomas
Dec 1, 1991·The Journal of Allergy and Clinical Immunology·L Puerta LlerenaR F Lockey
Jul 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L K ArrudaC K Naspitz
Nov 1, 1990·Allergy Proceedings : the Official Journal of Regional and State Allergy Societies·E Fernández-CaldasR F Lockey
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Dec 1, 1987·The Journal of Allergy and Clinical Immunology·T A Platts-Mills, M D Chapman
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Mar 1, 1982·Clinical Allergy·M GabrielD G Wraith
May 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H D ShenW R Thomas
Nov 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L CaraballoL Moreno
Sep 1, 1994·The Journal of Allergy and Clinical Immunology·B E StanalandR F Lockey
Apr 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E Fernández-CaldasL R Caraballo
Mar 1, 1993·Nature Genetics·W Gish, D J States
May 1, 1993·The Journal of Allergy and Clinical Immunology·L G ArlianE Fernandez-Caldas

❮ Previous
Next ❯

Citations

Mar 15, 2002·Current Allergy and Asthma Reports·L G Arlian
Jul 17, 2003·International Journal for Parasitology·K A HamiltonP F Billingsley
Dec 1, 2001·Annual Review of Entomology·Larry G Arlian
Oct 20, 2005·Journal of Bioscience and Bioengineering·Seiji KawamotoKazuhisa Ono
Nov 26, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Geoffrey A MuellerAnna Pomés
Apr 21, 2007·The Journal of Allergy and Clinical Immunology·Yun Feng GaoFook Tim Chew
Feb 20, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J ZakzukL Caraballo
Apr 4, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J SpiricR L Rabin
May 1, 2017·International Archives of Allergy and Immunology·Eduardo Santos da SilvaFatima Ferreira
Jun 26, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F T ChewB W Lee
Jun 10, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S Olsson, M van Hage-Hamsten
Oct 6, 2004·Allergy·E Fernández-Caldas, R F Lockey
Nov 2, 2012·Allergy, Asthma & Immunology Research·Kyoung Yong JeongChein-Soo Hong
Oct 27, 1998·Allergy·W R Thomas, W Smith
Apr 24, 2003·Allergy·N CheongK Y Chua
Apr 15, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J D A RamosK Y Chua
Jan 22, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G A Stewart, C Robinson
Jan 22, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C MoraA Díaz
Apr 12, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W R ThomasW Smith
Nov 17, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F C YiB W Lee
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.