Nucleotide sequence analysis of the HLA class I region spanning the 237-kb segment around the HLA-B and -C genes

Genomics
N MizukiH Inoko

Abstract

To elucidate the detailed gene organization of the human leukocyte antigen (HLA) class I region on chromosome 6, seven contiguous cosmid genomic clones covering the 237-kb segment around the HLA-B and -C loci were subjected to DNA sequencing by the shotgun strategy to give a single contig of 236,822 bp from the MICA gene (58.2 kb centromeric of HLA-B) to 90.8 kb telomeric of HLA-C. This region was confirmed to contain four known genes, MICA, HLA-17, HLA-B, and HLA-C, from centromere to telomere. Further, a new member of the P5 multicopy genes was found to be about 1.3 kb upstream of the HLA-17 gene and designated P5.8. Five novel genes designated NOB1-5 were identified by RT-PCR and Northern blot hybridization. In addition, two pseudogenes, dihydrofolate reductase pseudogene (DHFRP) and ribosomal protein L3 homologous gene (RPL3-Hom), were also found in the vicinity of the HLA-B and -C genes, respectively. The two segments (about 40 kb) downstream of the HLA-B and HLA-C genes showed high sequence homology to each other, suggesting that segmental genome duplication including the major histocompatibility complex (MHC) class I gene must have occurred during the evolution of the MHC.

References

Aug 31, 1992·Biochemical and Biophysical Research Communications·Y Kuwano, I G Wool
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K BronsonD D Chaplin
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M J ChenA W Nienhuis
Oct 1, 1994·Tissue Antigens·A Steinle, D J Schendel
Jul 1, 1993·Immunology Today·R D Campbell, J Trowsdale

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Citations

May 13, 1999·Immunological Reviews·T ShiinaH Inoko
May 13, 1999·Immunological Reviews·M Carrington
May 13, 1999·Immunological Reviews·Y SattaN Takahata
Dec 26, 2002·Annual Review of Immunology·Attila KumánovicsKirsten Fischer Lindahl
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kaoru Fukami-KobayashiYoshio Tateno
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ShiinaH Inoko
Jun 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya AnzaiHidetoshi Inoko
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·T GuillaudeuxD E Geraghty
Feb 13, 2001·Tissue Antigens·M Komatsu-WakuiT Juji
Sep 1, 1999·The Journal of Investigative Dermatology·M E Handel-FernandezV Vincek
Oct 6, 2005·Xenotransplantation·Douglas M SmithJeffrey M Asbury
Apr 2, 2014·Tissue Antigens·F PanW Tian
May 16, 2006·Human Immunology·Jan E Van AutreveUNKNOWN Belgian Diabetes Registry
Mar 22, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·A HampeF Galibert
Jul 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hugo G HiltonPeter Parham
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takashi ShiinaMarcia M Miller

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