Nucleotide sequence and analysis of the lethal factor gene (lef) from Bacillus anthracis

Gene
T S Bragg, D L Robertson

Abstract

The nucleotide sequence of the Bacillus anthracis lethal factor (LF) gene (lef) has been determined. LF is part of the tripartite protein exotoxin of B. anthracis along with protective antigen (PA) and edema factor (EF). The apparent ATG start codon, which is located immediately upstream from codons which specify the first 16 amino acids (aa) of the mature secreted LF, is preceded by an AAAGGAG sequence, which is its probable ribosome-binding site. This ATG codon begins a continuous 2427-bp open reading frame which encodes the 809-aa LF-precursor protein with an Mr of 93,798. The mature secreted protein (776 aa; Mr 90,237) was preceded by a 33-aa signal peptide which has characteristics in common with leader peptides for other secreted proteins of the Bacillus species. The codon usage of the LF gene reflects its high (70%) A + T content. The N-terminus of LF (first 300 aa) shared extensive homology with the N-terminus of the anthrax EF protein. Since LF and EF each bind PA at the same site, these homologous regions probably represent their common PA-binding domains.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·B H WadeG L Mandell
Nov 25, 1986·Nucleic Acids Research·R M MacKayV Seligy
Dec 16, 1987·Biochemical and Biophysical Research Communications·R L Kaspar, D L Robertson
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S H Leppla
Sep 1, 1983·Cell·M H Vodkin, S H Leppla
Feb 1, 1960·Journal of General Microbiology·J L STANLEYH SMITH
Sep 1, 1961·Journal of General Microbiology·J L STANLEY, H SMITH

❮ Previous
Next ❯

Citations

Jun 9, 2001·Biochemical and Biophysical Research Communications·P GuptaR Bhatnagar
Jul 27, 2001·Biochemical and Biophysical Research Communications·P GuptaR Bhatnagar
Jan 1, 1994·Molecular Aspects of Medicine·G MenestrinaC Montecucco
Nov 1, 1995·Research in Microbiology·J C SirardA Fouet
Oct 10, 2002·Biochemical and Biophysical Research Communications·Reetika GaurYogendra Singh
Feb 17, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·O RossettoC Montecucco
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·F Brossier, M Mock
Dec 14, 1999·Microbes and Infection·S F Little, B E Ivins
Mar 27, 2001·Letters in Applied Microbiology·Y H ShangkuanM F Shaio
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·A P Pugsley
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S R BlankeR J Collier
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·P J JacksonP Keim
Feb 11, 2009·The Journal of General Physiology·Daniel BasilioAlan Finkelstein
Jun 1, 2011·The Journal of General Physiology·Daniel BasilioAlan Finkelstein
Mar 18, 2000·Infection and Immunity·F BrossierJ C Sirard
May 24, 2005·Infection and Immunity·Janine M LamonicaVito G DelVecchio
Jan 2, 2004·Journal of Bacteriology·Melissa DrysdaleTheresa M Koehler
Oct 5, 2012·Journal of Clinical Microbiology·Leslie A DauphinAlex R Hoffmaster
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Holger BarthBradley G Stiles
Sep 24, 2008·BMC Infectious Diseases·Rasha HammamiehJohn Ezzell
Dec 2, 2005·Emerging Infectious Diseases·Tara WahabLars Engstrand
Jul 1, 2009·Molecular Aspects of Medicine·R John Collier
Mar 28, 2008·Molecular Microbiology·Justin W Kern, Olaf Schneewind
Nov 13, 2009·Molecular Microbiology·Justin Kern, Olaf Schneewind
Nov 4, 2004·Biosensors & Bioelectronics·James PannucciRobert B Cary
Nov 26, 2002·Biochemical and Biophysical Research Communications·Mohd Azhar AzizRakesh Bhatnagar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.