PMID: 2104607Jan 1, 1990Paper

Nucleotide sequence and cloning in Bacillus subtilis of the Bacillus stearothermophilus pleiotropic regulatory gene degT

Journal of Bacteriology
M TakagiT Imanaka

Abstract

The regulatory gene (degT) from Bacillus stearothermophilus NCA1503 which enhanced production of extracellular alkaline protease (Apr) was cloned in Bacillus subtilis with pTB53 as a vector. When B. subtilis MT-2 (Npr- [deficiency of neutral protease] Apr+) was transformed with the recombinant plasmid, pDT145, the plasmid carrier produced about three times more alkaline protease than did the wild-type strain. In contrast, when B. subtilis DB104 (Npr- Apr-) was used as a host, the transformant with pDT145 could not exhibit any protease activity. After construction of the deletion plasmids, DNA sequencing was done. A large open reading frame was found, and nucleotide sequence analysis showed that the degT gene was composed of 1,116 bases (372 amino acid residues, molecular weight of 41,244). A Shine-Dalgarno sequence was found nine bases upstream from the open reading frame. A B. subtilis strain carrying degT showed the following pleiotropic phenomena: (i) enhancement of production of extracellular enzymes such as alkaline protease and levansucrase, (ii) repression of autolysin activity, (iii) decrease of transformation efficiency for B. subtilis (competent cell procedure), (iv) altered control of sporulation, (v) loss of flagell...Continue Reading

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Citations

Feb 1, 1995·Molecular Microbiology·K M Ottemann, J J Mekalanos
Jul 12, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Tadayuki Imanaka, Haruyuki Atomi
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Jan 21, 2017·Molecular Oral Microbiology·M RangarajanM A Curtis
Jan 1, 1994·Critical Reviews in Biotechnology·W Piepersberg
Jun 5, 2002·FEMS Microbiology Letters·Christine JosenhansSebastian Suerbaum
Sep 24, 2004·The Journal of Biological Chemistry·Wei-Chun KaoSunney I Chan
Apr 15, 2000·Microbial Pathogenesis·T ChenM J Duncan
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Jun 15, 1992·Gene·J DistlerW Piepersberg
Nov 1, 1991·Journal of Bacteriology·A Kuroda, J Sekiguchi
Oct 1, 1991·Current Opinion in Biotechnology·R H Doi

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