PMID: 2120190Oct 1, 1990Paper

Nucleotide sequence and expression in Escherichia coli of the Lactococcus lactis citrate permease gene

Journal of Bacteriology
S DavidW M de Vos

Abstract

The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of proteins encoded by the cloned fragment in a T7 expression system revealed a 32,000-dalton protein band, which correlated with the ability of cells to transport citrate. Energy-dependent [1,5-14C]citrate transport was found with membrane vesicles prepared from E. coli cells harboring the citrate permease-expressing plasmid. The gene encoding citrate transport activity, citP, was located on the cloned fragment by introducing a site-specific mutation that abolished citrate transport and resulted in a truncated form of the 32,000-dalton expression product. The nucleotide sequence for a 2.2-kilobase fragment that includes the citP gene contained an open reading frame of 1,325 base pairs coding for a very hydrophobic protein of 442 amino acids, which shows no sequence homology with known citrate carriers.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1978·Archives of Biochemistry and Biophysics·W W Kay, M Cameron
Dec 1, 1978·Journal of General Microbiology·H W SmithP Green
Nov 1, 1979·Applied and Environmental Microbiology·N IshiguroG Sato
Feb 1, 1975·Journal of Bacteriology·C L JohnsonJ R Stern
Apr 30, 1990·European Journal of Biochemistry·M E van der RestW N Konings
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jun 1, 1987·Molecular & General Genetics : MGG·T NakaoY Anraku
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Feb 12, 1987·Nature·M C MaidenP J Henderson
Jan 1, 1985·Annual Review of Biochemistry·W R McClure
Jan 1, 1968·Journal of Bacteriology·R A Speckman, E B Collins
May 1, 1969·Journal of Bacteriology·R W O'Brien, J R Stern
Jan 1, 1981·Molecular & General Genetics : MGG·J M SomersW W Kay
Dec 1, 1983·Journal of Bacteriology·C H Reynolds, S Silver
Feb 7, 1980·Nature·D E BüchelB Müller-Hill
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Sep 1, 1980·Applied and Environmental Microbiology·N IshiguroG Sato
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
May 1, 1962·Journal of Bacteriology·R J HARVEY, E B COLLINS

❮ Previous
Next ❯

Citations

Feb 1, 1992·FEMS Microbiology Reviews·M van de GuchteG Venema
Oct 1, 1994·FEMS Microbiology Letters·F López de FelipeP López
Jan 7, 1998·FEMS Microbiology Letters·J M SantosC M Arraiano
Aug 3, 2006·Journal of Applied Microbiology·M NomuraT Okamoto
Oct 1, 1996·The Journal of Applied Bacteriology·R M de FigueroaG Oliver
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Dec 25, 2007·Applied and Environmental Microbiology·Claudia SánchezHelena Santos
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Iwona Sobczak, Juke S Lolkema
Mar 21, 1998·Applied and Environmental Microbiology·N García-QuintánsP López
Oct 27, 1995·The Journal of Biological Chemistry·C Marty-TeyssetW N Konings
Feb 1, 1995·Applied and Environmental Microbiology·N KlijnW M de Vos
Aug 30, 1994·Biochimica Et Biophysica Acta·J S LolkemaW N Konings
Jun 29, 1994·Biochimica Et Biophysica Acta·J ReizerM H Saier
Dec 1, 1991·Applied and Environmental Microbiology·M J Starrenburg, J Hugenholtz
Aug 1, 1992·Current Opinion in Cell Biology·J K GriffithP J Henderson
Sep 30, 2004·International Journal of Food Microbiology·Eveline J Bartowsky, Paul A Henschke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.