PMID: 7012834Dec 1, 1980Paper

Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor

Proceedings of the National Academy of Sciences of the United States of America
R P Gunsalus, C Yanofsky

Abstract

The nucleotide sequence of trpR of Escherichia coli was determined. This gene codes for a polypeptide (Mr 12,356) that is 108 amino acid residues in length. NH2-terminal, COOH-terminal, and total amino acid analyses of purified aporepressor agree with the deduced amino acid sequence and establish the translation start and stop codons of the structural gene. The transcription start site for trpR mRNA synthesis in vitro was shown to be 56 base pairs prior to the translation start site. The nucleotide sequence on either side of the transcription start site is homologous to the trp operon operator. Purified trp aporepressor, when activated by L-tryptophan, protects restriction sites in this region, the presumed trpR operator, from cleavage by the respective restriction endonucleases. Bound RNA polymerase protects the same restriction sites. These findings and the additional observation that trp repressor inhibits transcription initiation in vitro establish that there is a functional overlap of operator and promoter sequences in the regulatory region of the trpR operon. These findings indicate that expression of trpR is autoregulatory.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D L OxenderC Yanofsky
Nov 1, 1979·Molecular & General Genetics : MGG·W Roeder, R L Somerville
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
May 15, 1978·Journal of Molecular Biology·G N Bennett, C Yanofsky
Aug 24, 1978·Nature·P J Farabaugh
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A M Wu, T Platt
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L E PostP P Dennis
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B P Nichols, C Yanofsky
Mar 1, 1978·FEBS Letters·F Sanger, A R Coulson
Nov 16, 1978·Nature·R T Sauer
May 15, 1976·Journal of Molecular Biology·C SquiresC Yanofsky
Mar 6, 1975·Nature·J Shine, L Dalgarno
Feb 15, 1975·Journal of Molecular Biology·C L SquiresC Yanofsky
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G ZubayJ H Miller
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J K Rose, C Yanofsky
Aug 28, 1969·Journal of Molecular Biology·D E Morse, C Yanofsky
Nov 15, 1971·JAMA : the Journal of the American Medical Association·C Yanofsky

❮ Previous
Next ❯

Citations

Aug 1, 1993·Electrophoresis·D E Lewis, J Carey
Sep 1, 1992·Protein Science : a Publication of the Protein Society·A E Howard, P A Kollman
Nov 1, 1993·Protein Science : a Publication of the Protein Society·C J MannC R Matthews
Aug 1, 1994·Protein Science : a Publication of the Protein Society·J GuenotP A Kollman
Jan 24, 1998·Biochemical and Biophysical Research Communications·C FentonM R el-Gewely
Jan 1, 1985·Molecular & General Genetics : MGG·P RitzenthalerM Mata-Gilsinger
Jan 1, 1983·Molecular & General Genetics : MGG·G Bogosian, R Somerville
Jan 1, 1984·Molecular & General Genetics : MGG·H GrosfeldA Shafferman
Jan 1, 1985·Molecular & General Genetics : MGG·M G Marinus
Jun 12, 1996·Molecular & General Genetics : MGG·C Günes, B Müller-Hill
Feb 22, 2011·Applied Microbiology and Biotechnology·Thomas DrepperKarl-Erich Jaeger
May 5, 2011·Journal of Industrial Microbiology & Biotechnology·Zhi-Jun ZhaoJian Chen
Oct 7, 1985·FEBS Letters·J F LefevreO Jardetzky
Apr 6, 1990·Biochimica Et Biophysica Acta·B F Luisi, P B Sigler
Nov 20, 1992·Biochimica Et Biophysica Acta·J N EvansM F Roberts
Aug 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Devin StricklandTobin R Sosnick
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R Brent, M Ptashne
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R L Kelley, C Yanofsky
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A JoachimiakP B Sigler
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R L Kelley, C Yanofsky
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U DeuschleH Bujard
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D B LimW K Maas
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S J BaeJ M Sturtevant
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Carey
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M Wurgler, C C Richardson
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C BurgeS Karlin
Aug 25, 1982·Nucleic Acids Research·I T WeberT A Steitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.