PMID: 2105307Feb 1, 1990Paper

Nucleotide sequence and further characterization of the Synechococcus sp. strain PCC 7002 recA gene: complementation of a cyanobacterial recA mutation by the Escherichia coli recA gene

Journal of Bacteriology
R C MurphyR D Porter

Abstract

The nucleotide sequence and transcript initiation site of the Synechococcus sp. strain PCC 7002 recA gene have been determined. The deduced amino acid sequence of the RecA protein of this cyanobacterium is 56% identical and 73% similar to the Escherichia coli RecA protein. Northern (RNA) blot analysis indicates that the Synechococcus strain PCC 7002 recA gene is transcribed as a monocistronic transcript 1,200 bases in length. The 5' endpoint of the recA mRNA was mapped by primer extension by using synthetic oligonucleotides of 17 and 27 nucleotides as primers. The nucleotide sequence 5' to the mapped endpoint contained sequence motifs bearing a striking resemblance to the heat shock (sigma 32-specific) promoters of E. coli but did not contain sequences similar to the E. coli SOS operator recognized by the LexA repressor. An insertion mutation introduced into the recA locus of Synechococcus strain PCC 7002 via homologous recombination resulted in the formation of diploids carrying both mutant and wild-type recA alleles. A variety of growth regimens and transformation procedures failed to produce a recA Synechococcus strain PCC 7002 mutant. However, introduction into these diploid cells of the E. coli recA gene in trans on a biph...Continue Reading

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Citations

Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·A I Roca, M M Cox
Aug 26, 2014·Frontiers in Bioengineering and Biotechnology·Patrik R Jones
Jan 18, 2015·Photosynthesis Research·Jeffrey C CameronBrian F Pfleger
Nov 25, 2016·Frontiers in Microbiology·Corinne Cassier-ChauvatFranck Chauvat
Mar 8, 1994·Biochimica Et Biophysica Acta·H LillN Nelson
Jan 2, 1992·Gene·M E TolmaskyJ H Crosa
Jun 1, 1995·Journal of Bacteriology·C MackenzieG M Weinstock

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