PMID: 2492509Feb 1, 1989Paper

Nucleotide sequence and transcriptional analysis of the Streptomyces griseus gene (afsA) responsible for A-factor biosynthesis

Journal of Bacteriology
S HorinouchiT Beppu

Abstract

The nucleotide sequence of the Streptomyces griseus afsA gene, possibly encoding a key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) biosynthesis, was determined. The translational initiation codon was identified by introducing out-of-frame mutations at appropriate positions by oligonucleotide-directed mutagenesis. The afsA gene was thus found to code for a protein of 301 amino acid residues and 32.6 kilodaltons whose codon usage pattern was in agreement with the general tendency of Streptomyces genes with an extremely high guanine-plus-cytosine content. High-resolution S1 nuclease mapping indicated that the transcriptional start point was the A residue, the first position of the ATG translational initiation codon.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1985·The Journal of Antibiotics·S HorinouchiT Beppu
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Jan 15, 1980·Analytical Biochemistry·C W Chen, C A Thomas
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Sep 1, 1982·The Journal of Antibiotics·O Hara, T Beppu
Jan 1, 1983·Methods in Enzymology·J Messing
Dec 1, 1982·The Journal of Antibiotics·U GräfeL Radics
Jun 1, 1980·Microbiological Reviews·J F Martin, A L Demain

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Citations

Jan 1, 1990·Critical Reviews in Biotechnology·S Horinouchi, T Beppu
Dec 1, 1991·Molecular Microbiology·B K LeskiwK F Chater
Dec 29, 2004·FEMS Microbiology Letters·T V N Ramachander, S K Rawal
Aug 30, 2008·Science in China. Series C, Life Sciences·G Shang, Y Wang
Apr 1, 1991·Journal of Industrial Microbiology·W R StrohlR H Woodman
Sep 11, 1999·Journal of Bacteriology·D KameokaH Kinashi
Mar 1, 1996·Biochimica Et Biophysica Acta·M HaapalainenM C Metzler
Aug 1, 1989·Journal of Bacteriology·K MiyakeT Beppu
Nov 1, 1995·Journal of Bacteriology·A LezhavaH Kinashi

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