Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli

Gene
J S Miles, J R Guest

Abstract

A 1.6-kb MspI-HindIII chromosomal DNA segment, carrying the complete coding region of the 6-phosphomannose isomerase (PMI) structural gene, manA, and the 5' end of the gene encoding the major fumarase activity, fumA, of Escherichia coli K-12, has been sequenced using the chain termination method. The start points of manA and fumA transcripts were located by S1 mapping using 32P-labelled M13 ssDNA probes, and the two genes were shown to be transcribed divergently. The sequence of the 390 amino acid residues comprising the PMI monomer has been deduced, and the predicted Mr of 42 716 agrees with that for the protein of Mr 42 000 identified previously by the maxicell procedure.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 11, 1979·Nucleic Acids Research·R Staden
Jun 1, 1973·Journal of Bacteriology·M G Marinus, N R Morris
Aug 5, 1967·Nature·W SalserA Bolle
Jun 26, 1981·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A AchariE A Noltmann
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Dec 25, 1982·Journal of Molecular Biology·F SangerG B Petersen
Jan 15, 1980·Analytical Biochemistry·C W Chen, C A Thomas
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Dec 21, 1981·Nucleic Acids Research·C SquiresC L Squires
Feb 1, 1983·Journal of Bacteriology·J R Guest, R E Roberts
Dec 1, 1983·Journal of Bacteriology·C Chapon, A Kolb
Apr 25, 1984·Nucleic Acids Research·J S Miles, J R Guest
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Jun 1, 1983·Microbiological Reviews·B J Bachmann
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
May 11, 1982·Nucleic Acids Research·G D StormoL M Gold
May 11, 1982·Nucleic Acids Research·G D StormoA Ehrenfeucht
Jan 11, 1982·Nucleic Acids Research·R Staden, A D McLachlan
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jan 1, 1980·Methods in Enzymology·H O Smith
Feb 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·A MARKOVITZ

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Citations

Oct 1, 1994·Journal of Protein Chemistry·A E ProudfootT N Wells
Mar 28, 2006·Plant Cell Reports·Zhengquan HeJianwu Dai
Jun 14, 2006·Plant Cell Reports·Juliana DegenhardtIris Szankowski
Mar 21, 1998·Biochimica Et Biophysica Acta·S O Jensen, P R Reeves
May 23, 2013·Journal of Agricultural and Food Chemistry·Tristan E LipkieMario G Ferruzzi
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Aug 30, 2008·The Journal of Biological Chemistry·Takanori MarutaShigeru Shigeoka
Apr 11, 1993·Nucleic Acids Research·S Lisser, H Margalit
Jan 15, 1994·European Journal of Biochemistry·A E ProudfootD J Smith
Aug 12, 2005·Genome Génome / Conseil National De Recherches Canada·Daolin FuGeorge H Liang
Sep 16, 2008·Journal of Plant Physiology·Isaac Kirubakaran Sundar, Natarajan Sakthivel
Mar 20, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bryan DelaneyUNKNOWN ILSI International Food Biotechnology Committee Task Force on Protein Safety
Dec 7, 2006·Plant Biotechnology Journal·Zhensheng GaoGeorge H Liang
Feb 21, 2007·Plant Biotechnology Journal·Ashok Kumar Shrawat, Horst Lörz
Jul 27, 2001·Microbial & Comparative Genomics·M H Serres, M Riley
Mar 24, 2015·Molecular Breeding : New Strategies in Plant Improvement·Muqing ZhangRukai Chen
Sep 5, 1989·Biochemistry·G K FarberG A Petsko
Jan 1, 2014·In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association·Emily WuZuo-Yu Zhao

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