Nucleotide sequence of a Bacillus subtilis arginine regulatory gene and homology of its product to the Escherichia coli arginine repressor

Gene
A K NorthS Baumberg

Abstract

In Bacillus subtilis, arginine represses its biosynthetic enzymes and activates its catabolic ones via a regulator gene ahrC. A 6.2-kb EcoRI fragment of B. subtilis chromosomal DNA that includes the ahrC gene has previously been cloned. Gene ahrC was localised in a 0.8-kb HindIII sub-fragment whose nucleotide sequence was determined. An open reading frame (ORF) was present whose translated amino acid sequence showed homology to that of the Escherichia coli arginine repressor encoded by that organism's argR gene. That this ORF corresponded to ahrC was confirmed by (i) the location of the transposon in an ahrC::Tn917 insertion mutant within the ORF; and (ii) by the appearance of an AhrC- phenotype when plasmids carrying restriction fragments lying wholly within this ORF were permitted to integrate by Campbell-type recombination into the B. subtilis chromosome. This represents the first description of a repressor in a 'housekeeping' biosynthetic system in a Bacillus, and indeed of homology between regulatory proteins for any 'housekeeping' system across such a wide taxonomic barrier among prokaryotes.

References

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Citations

Sep 18, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Z SekeyováD Raoult
Nov 17, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·James A GarnettSimon E V Phillips
Nov 17, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·James A GarnettSimon E V Phillips
Mar 10, 2012·PloS One·Rosario Pérez-RedondoPaloma Liras
Jul 30, 2014·Extremophiles : Life Under Extreme Conditions·Naoki IwanagaMakoto Nishiyama
May 6, 2008·Journal of Molecular Biology·James A GarnettSimon E V Phillips
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Aug 1, 1994·Molecular Microbiology·G Tian, W K Maas
Mar 1, 1990·Journal of Bacteriology·B E Van Hoy, J A Hoch

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