Nucleotide sequence of a Madagascar hepatopancreatic parvovirus (HPV) and comparison of genetic variation among geographic isolates

Diseases of Aquatic Organisms
Kathy F J TangDonald V Lightner

Abstract

A segment of Madagascar hepatopancreatic parvovirus (HPV) genomic sequence (5742 nucleotides) was determined through PCR and direct sequencing. This nucleotide sequence was compared to isolates from Australia, Thailand, Korea, and Tanzania, and the mean distance was determined to be 17%. The Madagascar HPV is closest to the Tanzania isolate (12%), followed by isolates from Korea (15%), Australia (17%) and Thailand (20%). Analysis of the genomic structure revealed that this HPV sequence is comprised of one partial Left open reading frame (ORF) (349 amino acids, aa) and complete Mid (578 aa) and Right (820 aa) ORFs. The amino acid sequences of the 3 ORFs were compared among isolates. The Right ORF was found to have the highest variation with a mean distance of 24%. This was followed by the Left and Mid ORF with distances of 13 and 7%, respectively. A phylogenetic analysis based on the amino acid sequence of the Right ORF divides 7 HPV isolates into 3 well-separated groups: Korea, Thailand, and Australia. The Madagascar HPV clustered with the Korea and Tanzania isolates. In Madagascar, HPV has been detected by histological examination since the 1990s. PCR analysis of a recent (2007) sampling showed a 100% prevalence. HPV was also ...Continue Reading

Citations

Sep 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Muhammed P SafeenaIndrani Karunasagar
Oct 5, 2013·Journal of Invertebrate Pathology·Tianqi LiuJie Huang
Feb 17, 2010·Virus Research·Muhammed P SafeenaIndrani Karunasagar
Feb 6, 2017·Journal of Invertebrate Pathology·K S Bateman, G D Stentiford
Feb 26, 2014·World Journal of Virology·Parin ChaivisuthangkuraPaisarn Sithigorngul
May 31, 2019·Virusdisease·Arun K DharDarryl Jory
Aug 30, 2018·Diseases of Aquatic Organisms·Sudini FernandoRapeepun Vanichviriyakit
Dec 12, 2018·Microbiology Resource Announcements·Mohamed E MegahedArun K Dhar
Aug 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Judit J PénzesPeter Tijssen

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