PMID: 11913793Mar 27, 2002Paper

Nucleotide sequence of envelope protein of Japanese encephalitis virus SA14-14-2 adapted to vero cells

DNA Sequence : the Journal of DNA Sequencing and Mapping
S P HongS O Kim

Abstract

Live attenuated Japanese encephalitis (JE) virus SA(14)-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA(14)-14-2(Vero). Animal testing showed that SA(14)-14-2(Vero) has an attenuation phenotype similar to that of the parent SA(14)-14-2(PDK) strain in mice.

References

Apr 27, 1991·Lancet·M M Andersen, T Rønne
Jan 1, 1986·Advances in Virus Research·F X Heinz
Jul 1, 1988·Journal of Virology·S LustigJ H Strauss
Jun 8, 1996·Lancet·M C Steinhoff

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