PMID: 6990415Apr 1, 1980Paper

Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
F E NargangH E Umbarger

Abstract

The nucleotide sequence of the DNA thought to contain the control region for the ilvGEDA operon in Escherichia coli has been determined by the Maxam-Gilbert procedure. The sequence includes a region that, upon transcription, would yield a leader transcript specifying a peptide 32 residues long. This putative peptide would contain four leucine, five isoleucine, and six valine residues. A model is proposed that correlates the multivalent control of the ilvGEDA operon with the extent to which this leader transcript is translated. In vitro transcription experiments yielded a transcript of about 183 nucelotides, compatible with the predictions of the model.

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Jan 1, 1983·Molecular & General Genetics : MGG·G Bogosian, R Somerville
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