Nucleotide sequence of the celC gene encoding endoglucanase C of Clostridium thermocellum

Gene
W H SchwarzW L Staudenbauer

Abstract

The nucleotide sequence of the cellulase gene celC, encoding endoglucanase C of Clostridium thermocellum, has been determined. The coding region of 1032 bp was identified by comparison with the N-terminal amino acid (aa) sequence of endoglucanase C purified from Escherichia coli. The ATG start codon is preceded by an AGGAGG sequence typical of ribosome-binding sites in Gram-positive bacteria. The derived amino acid sequence corresponds to a protein of Mr 40,439. Amino acid analysis and apparent Mr of endoglucanase C are consistent with the amino acid sequence as derived from the DNA sequencing data. A proposed N-terminal 21-aa residue leader (signal) sequence differs from other prokaryotic signal peptides and is non-functional in E. coli. Most of the protein bears no resemblance to the endoglucanases A, B, and D of the same organism. However, a short region of homology between endoglucanases A and C was identified, which is similar to the established active sites of lysozymes and to related sequences of fungal cellulases.

References

Jul 1, 1987·Analytical Biochemistry·W H SchwarzW L Staudenbauer
Nov 25, 1986·Nucleic Acids Research·R M MacKayV Seligy
Feb 25, 1986·Nucleic Acids Research·O Grépinet, P Béguin
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Apr 1, 1985·Journal of Bacteriology·P BéguinJ P Aubert
Jun 3, 1985·European Journal of Biochemistry·A J Clarke, M Yaguchi
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Oct 31, 1983·Biochemical and Biophysical Research Communications·M YaguchiL Jurasek
Mar 26, 1984·Nucleic Acids Research·P H Van KnippenbergH A Heus
Jun 1, 1983·European Journal of Biochemistry·G von Heijne
Jan 1, 1981·Annual Review of Biochemistry·G Kreil
Jun 1, 1986·Applied and Environmental Microbiology·W H SchwarzW L Staudenbauer

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Citations

Jan 1, 1993·Applied Biochemistry and Biotechnology·W K Wang, J H Wu
Feb 15, 2011·Applied Microbiology and Biotechnology·Michael NewcombJ H David Wu
Dec 1, 1989·FEMS Microbiology Reviews·M YoungW L Staudenbauer
Oct 19, 1995·Biochimica Et Biophysica Acta·R MacarrónM Claeyssens
Sep 1, 1989·Gene·B HenrissatJ P Mornon
Jan 1, 1989·Biotechnology Advances·B R Glick, J J Pasternak
Nov 1, 1991·Research in Microbiology·A NavarroJ P Aubert
Feb 1, 1992·Biochemical Society Transactions·P BéguinM K Daniel
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Arnold L DemainJ H David Wu
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael NewcombJ H David Wu
Sep 26, 1988·FEBS Letters·P RoggentinR Schauer
Feb 6, 2004·Molecular Microbiology·Stéphanie PerretChantal Tardif
Aug 3, 2011·Biotechnology and Bioengineering·Somayesadat BadieyanChenming Zhang
Dec 16, 1991·Biochemical and Biophysical Research Communications·V V ZverlovG A Velikodvorskaja
Mar 30, 1990·Biochemical and Biophysical Research Communications·B K Bumazkin Strongin AYa
May 23, 2015·Applied Microbiology and Biotechnology·Elizabeth B MearlsLee R Lynd
Jan 1, 1989·Critical Reviews in Biotechnology·V S Bisaria, S Mishra
Dec 15, 1992·FEMS Microbiology Letters·P BéguinJ P Aubert
Oct 1, 1989·Molecular & General Genetics : MGG·E BergerD R Woods
Nov 1, 1994·Applied Microbiology and Biotechnology·W K WangJ H Wu
Dec 1, 1989·Journal of Bacteriology·K OhmiyaS Shimizu
Oct 1, 1990·Applied and Environmental Microbiology·T KobayashiA L Demain
Jul 1, 1990·Journal of Bacteriology·O MatsushitaD B Wilson
Jan 1, 1991·Journal of Bacteriology·S MishraJ P Aubert

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