PMID: 6159575May 24, 1980Paper

Nucleotide sequence of the E coli gene coding for dihydrofolate reductase

Nucleic Acids Research
D R Smith, J M Calvo

Abstract

Plasmid pLC1437a contains DNA from Escherichia coli K12 including fol, the structural gene for dihydrofolate reductase. The fol gene was mapped on this plasmid relative to several restriction endonuclease cleavage sites. fol was also cloned from strain RSO and the nucleotide sequence for the entire fol gene and its flanking regions from this strain was determined. The amino acid sequence predicted from the nucleotide sequence differs in only a few respects from the reported amino acid sequence of dihydrofolate reductase from E. coli B. The major RNA transcripts initiated at the fol promotor in vivo are approximately 550 and 590 nucleotides long. In addition to these, several longer transcripts (up to 1400 nucleotides) are present in lesser amounts. A new procedure is described for 3' end labeling of DNA fragments having blunt ends using E. coli exonuclease III and avian myeloblastoma virus reverse transcriptase.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Mar 25, 1978·Journal of Molecular Biology·B S Zain, R J Roberts
Mar 7, 1977·Molecular & General Genetics : MGG·R Sheldon
Aug 1, 1979·Molecular & General Genetics : MGG·D R Smith, J M Calvo
Aug 10, 1976·Molecular & General Genetics : MGG·R Sheldon, S Brenner
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Pribnow
Jun 1, 1975·Genetical Research·A S BreezeK A Stacey
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Jul 15, 1974·Journal of Molecular Biology·P N BorerO C Uhlenbeck
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla

❮ Previous
Next ❯

Citations

Jan 1, 1985·Molecular & General Genetics : MGG·D FriedbergJ M Calvo
Mar 1, 1989·Molecular & General Genetics : MGG·A LersA Zamir
May 1, 1984·Current Genetics·K Nath, E W Baptist
Oct 1, 1984·Molecular and Biochemical Parasitology·B J JohnsonJ E Donelson
Apr 1, 1982·Gene·T F DonahueG R Fink
Jan 1, 1986·Gene·A M Cigan, T F Donahue
Feb 19, 2002·Molecular and Biochemical Parasitology·Sudsanguan ChusacultanachaiYongyuth Yuthavong
Jan 13, 2000·European Journal of Biochemistry·V K WalkerR Chaguturu
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J B DodgsonJ D Engel
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R W Hackett, J T Lis
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C M Rice, J H Strauss
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C M CumminsM R Culbertson
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C D PauzaH K Schachman
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F J del ReyG R Fink
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M BallivetS Heinemann
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G M CannM E Koshland
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J ChoeM Grunstein
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·Z Livneh
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S S Monroe, S Schlesinger
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J Gearhart, D F Bogenhagen
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M I LermanM F Singer
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J Lammers, R Haselkorn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.