Nucleotide sequence of the Escherichia coli solA gene encoding a sarcosine oxidase-like protein and characterization of its product

Gene
Y Koyama, H Ohmori

Abstract

An Escherichia coli gene, designated solA, was identified between pyrC and htrB around 24.2 min of the genetic map. The solA gene product (SolA, 372 aa) shows strong similarities (at most 42% identities over the entire length) to monomeric sarcosine (N-methylglycine) oxidases from other bacteria. However, only low levels of sarcosine oxidase activity were detected, even when SolA was overproduced. The SolA extracts exhibited much higher (about 200-fold) reactivity toward N-methyltryptophan than toward sarcosine. Thus, SolA appears to have a substrate specificity distinct from that of monomeric sarcosine oxidases, in spite of the strong similarities in the primary structure with such enzymes.

References

Mar 1, 1992·Bioscience, Biotechnology, and Biochemistry·K SuzukiS Imamura
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Sep 30, 1980·Biochemical and Biophysical Research Communications·S HayashiM Suzuki

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Citations

Jun 10, 2008·Journal of Bacteriology·Kerstin HelbigDietrich H Nies
Feb 23, 2005·Biochemistry·Erik C Ralph, Paul F Fitzpatrick
Aug 29, 2006·Journal of Molecular Biology·Robert M G HynsonMarilyn Schuman Jorns
Jun 1, 2005·Protein Expression and Purification·Alshaimaa Hassan-AbdallahMarilyn Schuman Jorns
Dec 10, 2016·Scientific Reports·Katie J DenbyJeffrey Green
Dec 21, 2014·Applied and Environmental Microbiology·Anthony G DodgeMichael J Sadowsky
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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