PMID: 6985485Aug 11, 1982Paper

Nucleotide sequence of the gene encoding adenovirus type 2 DNA binding protein

Nucleic Acids Research
W KruijerJ S Sussenbach

Abstract

We have determined the nucleotide sequence of the gene encoding adenovirus type 2 (Ad2) DNA binding protein (DBP). From the nucleotide sequence the complete amino acid sequence of Ad2 DBP has been deduced. A comparison of the amino acid sequences of Ad2 and Ad5 DBP, both 529 residues long, reveals that the C-terminal 354 residues of both sequences are identical. Within the N-terminal 175 amino acid residues Ad2 and Ad5 show nine differences. The site of mutation in Ad2 ND1ts23, a mutant with a temperature-sensitive DNA replication, was mapped at the nucleotide level. A single nucleotide alteration in the DBP gene, resulting in a leucine leads to phenylalanine substitution at position 282 in the amino acid sequence is responsible for the temperature-sensitive character of this mutant. Previously, we localized the mutation of another DBP mutant with a temperature-sensitive DNA replication (H5ts125) at position 413 in the amino acid sequence of the DBP molecule (Nucleic Acids Res. 9 (1981) 4439-4457). These mapping data are discussed in relation to the structure and function of the DBP molecule.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G R KitchingmanH Westphal
Oct 25, 1979·Journal of Molecular Biology·L T ChowJ B Lewis
Jul 1, 1978·Cell·A J Berk, P A Sharp
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Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J R Nevins, J J Winkler

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Citations

Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M BehmM Bina
Nov 26, 1984·Nucleic Acids Research·S H Munroe
Mar 11, 1985·Nucleic Acids Research·B MignotteJ C Mounolou

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