Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the alpha-amylase gene from Bacillus amyloliquefaciens

Gene
I PalvaL Kääriäinen

Abstract

We have isolated and partially sequenced the gene coding for alpha-amylase (EC 3.2.1.1) from Bacillus amyloliquefaciens by molecular cloning in the plasmid pUB110 using Bacillus subtilis as a host. The nucleotide sequence of the NH2-terminal region of the cloned gene was determined and found to contain a 31-residue-long stretch of amino acids preceding the NH2-terminal sequence of the extracellular alpha-amylase. Within this sequence there is a 15-residue-long stretch of uncharged amino acids similar to that found at the NH2 terminus of other precursors to exported proteins. This "signal sequence" is probably removed in conjunction with the translocation of alpha-amylase through the cytoplasmic membrane. In vitro labeling of alpha-amylase with radioactive amino acids in a coupled transcription-translation system followed by partial sequencing established the exact location of the NH2 terminus of the alpha-amylase gene. The nucleotide sequence preceding the NH2 terminus has properties resembling the RNA-polymerase- and ribosome-binding sites found at the 5' terminus of many prokaryotic genes.

References

Dec 25, 1978·Journal of Molecular Biology·K SimonsA Helenius
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S InouyeM Inouye
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H Inouye, J Beckwith
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L L RandallL G Josefsson
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Mar 1, 1978·FEBS Letters·F Sanger, A R Coulson
Jul 1, 1978·Nucleic Acids Research·H F Tabak, R A Flavell
Jul 8, 1975·Biochemical and Biophysical Research Communications·L Fernández-Rivera Río, A Arroyo-Begovich
May 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R RadloffJ Vinograd
Feb 1, 1980·The Biochemical Journal·H Chung, F Friedberg
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J HedgpethM Hofnung
Jan 1, 1980·Molecular & General Genetics : MGG·G LeeJ Pero
Jan 31, 1980·Nature·B D Davis, P C Tai
Jan 1, 1980·European Journal of Biochemistry·N KalkkinenL Kääriäinen
Dec 1, 1980·European Journal of Biochemistry·E FuchsG Kälberer

❮ Previous
Next ❯

Citations

Jan 1, 1984·Zeitschrift für allgemeine Mikrobiologie·R WambuttM Schultze
Jul 1, 1991·Journal of Biotechnology·J VehmaanperäJ Hofemeister
Feb 1, 1985·Virus Research·K LundströmR F Pettersson
Sep 1, 1981·Gene·A Jalanko Söderlund
May 1, 1983·Gene·I PalvaC Weissmann
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I PalvaL Kääriäinen
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S LöfdahlM Lindberg
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·R H Doi
Jan 25, 1983·Nucleic Acids Research·M YangD Henner
Nov 25, 1983·Nucleic Acids Research·T Sako, N Tsuchida
Jul 11, 1984·Nucleic Acids Research·M E Watson
May 1, 1991·Molecular Microbiology·V P KontinenM Sarvas
Dec 17, 1984·European Journal of Biochemistry·M Sibakov, I Palva
Feb 24, 2006·Infection and Immunity·Brian K Janes, Scott Stibitz
Jul 3, 2002·Bioscience, Biotechnology, and Biochemistry·Hajime AgaYoshio Tsujisaka
Jan 1, 1986·Annals of the New York Academy of Sciences·D J McConnellP M Sharp
Jan 1, 1983·Annals of the New York Academy of Sciences·L C Klotz
Jan 1, 1983·Annals of the New York Academy of Sciences·S AibaJ Koizumi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.