PMID: 2121716Nov 1, 1990Paper

Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes

Journal of Bacteriology
L IsakiH C Wu

Abstract

The lsp gene encoding prolipoprotein signal peptidase (signal peptidase II) is organized into an operon consisting of ileS and three open reading frames, designated genes x, orf149, and orf316 in both Escherichia coli and Enterobacter aerogenes. A plasmid, pBROC128, containing a 5.8-kb fragment of Pseudomonas fluorescens DNA was found to confer pseudomonic acid resistance on E. coli host cells and to contain the structural gene of ileS from P. fluorescens. In addition, E. coli strains carrying pBROC128 exhibited increased globomycin resistance. This indicated that the P. fluorescens lsp gene was present on the plasmid. The nucleotide sequences of the P. fluorescens lsp gene and of its flanking regions were determined. Comparison of the nucleotide sequences of the lsp genes in E. coli and P. fluorescens revealed two highly conserved domains in this enzyme. Furthermore, the five genes which constitute an operon in E. coli and Enterobacter aerogenes were found in P. fluorescens in the same order as in the first two species.

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Citations

Oct 1, 1995·Molecular Membrane Biology·S L Rusch, D A Kendall
Oct 16, 1999·FEMS Microbiology Letters·A GuptaG N Qazi
Jun 20, 1997·The Journal of Biological Chemistry·S HottenrottJ U Rahfeld
Jan 7, 1998·FEMS Microbiology Letters·M M WöstenB A van der Zeijst
Sep 25, 1999·The Journal of Biological Chemistry·H TjalsmaJ M van Dijl
Apr 1, 1997·Microbiology·Zoltán PrágaiSierd Bron
Nov 7, 1995·Gene·T S Jones, V V Tryon
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Sep 29, 2006·Research in Microbiology·Nick GeukensElke Lammertyn

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