Nucleotide sequences of the hepatitis C virus core region in patients without anti-core antibody

Journal of Medical Virology
A NagasakaM Asaka

Abstract

Second-generation assays for detection of hepatitis C virus (HCV) infection that include reactivity of antibodies to core, NS3, NS4 are used because of their high sensitivity. Among these antibodies, anti-core antibody seems to be the most sensitive. However, there are some patients without anti-core antibodies, although HCV RNA is detectable by reverse transcription-polymerase chain reaction and branched DNA assay. The mechanism for the absence of anti-core antibody on its own is unclear. We therefore determined the nucleotide and deduced amino acid sequences of the core region obtained from two anti-core antibody-negative patients with HCV RNA (genotype 1b) and compared them with those of four anti-core antibody-positive patients and a previously reported sequence. Amino acids spanning 1-47, which seemed to exist in major B cell epitopes, were found to be completely conserved among these patients. Furthermore, the predictive binding motif to HLA DR4 (a.a 81-90) was completely conserved in both of the anti-core antibody-negative patients. There were various mutations in the residual amino acids spanning 49-108, but specific mutations could not be found in anti-core antibody-negative patients. These data indicate that the absen...Continue Reading

References

Jan 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J G McHutchisonR DiNello
Feb 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L J JeffersM Houghton
Feb 8, 1992·Lancet·D BoudartJ Y Muller
Aug 1, 1992·Journal of Clinical Microbiology·M SällbergL O Magnius
Nov 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A S LokJ S Lam
Oct 19, 1991·Lancet·E A FollettP Simmonds
Oct 19, 1991·Lancet·C Stornello
Jun 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N YukiT Kamada

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