Nucleotide sequences within the U5 region of the viral RNA genome are the major determinants for an human immunodeficiency virus type 1 to maintain a primer binding site complementary to tRNA(His)

Virology
Z ZhangC D Morrow

Abstract

The initiation of reverse transcription of the human immunodeficiency virus type 1 (HIV-1) genome requires cellular tRNA(Lys,3) as a primer and occurs at a site in the viral RNA genome, designated as the primer binding site (PBS), which is complementary to the 3'-terminal 18 nucleotides of tRNA(Lys,3). We previously described an HIV-1 virus [designated as HXB2(His-AC)], which contained a sequence within the U5 region complementary to the anticodon region of tRNA(His) in addition to a PBS complementary to the 3'-terminal 18 nucleotides of the tRNA(His). That virus maintained a PBS complementary to tRNA(His) after extended in vitro culture (Wakefield et al., J. Virol. 70, 966-975, 1996). In the present study, we report that subcloning a 200-base-pair DNA fragment encompassing the U5 and PBS regions from an integrated provirus of HXB2(His-AC) back into the wild-type genome (pHXB2) resulted in an infectious virus, designated as HXB2(His-AC-gac), which again stably maintained a PBS complementary to tRNA(His). DNA sequence analysis of the 200-base-pair region revealed only three nucleotide changes from HXB2(His-AC): a T-to-G change at nucleotide 174, a G-to-A change at nucleotide 181, and a T-to-C change at nucleotide 200. The new mu...Continue Reading

Citations

Apr 4, 1998·Nucleic Acids Research·C IselR Marquet
May 4, 2006·Nucleic Acids Research·Pavlina KonstantinovaBen Berkhout
Jul 30, 2003·Journal of Virology·Nathan J KellyCasey D Morrow
Nov 30, 2002·Biochimie·Christine BrunelChantal Ehresmann
Jan 12, 2002·Reviews in Medical Virology·David Harrich, Bill Hooker
Jun 15, 2004·The Journal of Biological Chemistry·Valérie GoldschmidtRoland Marquet
Jan 1, 2010·Viruses·Catherine IselRoland Marquet
Aug 27, 2002·The Journal of Biological Chemistry·Valerie GoldschmidtRoland Marquet
May 16, 2000·The Journal of Biological Chemistry·N Beerens, B Berkhout
Dec 31, 2020·International Journal of Molecular Sciences·Thomas GremmingerXiao Heng

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