Nucleotide variability and translation efficiency of the 5' untranslated region of hepatitis A virus: update from clinical isolates associated with mild and severe hepatitis.

Journal of Virology
Vincent MackiewiczAnne-Marie Roque-Afonso

Abstract

Mutations in the internal ribosome entry site (IRES) of hepatitis A virus (HAV) have been associated with enhanced in vitro replication and viral attenuation in animal models. To address the possible role of IRES variability in clinical presentation, IRES sequences were obtained from HAV isolates associated with benign (n = 8) or severe (n = 4) hepatitis. IRES activity was assessed using a bicistronic dual-luciferase expression system in adenocarcinoma (HeLa) and hepatoma (HuH7) cell lines. Activity was higher in HuH7 than in HeLa cells, except for an infrequently isolated genotype IIA strain. Though globally low, significant variation in IRES-dependent translation efficiency was observed between field isolates, reflecting the low but significant genetic variability of this region (94.2% +/- 0.5% nucleotide identity). No mutation was exclusive of benign or severe hepatitis, and variations in IRES activity were not associated with a clinical phenotype, indirectly supporting the preponderance of host factors in determining the clinical presentation.

References

Jun 1, 1992·The Journal of General Virology·B H RobertsonK Ito
Aug 1, 1989·The Journal of Infectious Diseases·A VallbrachtB Fleischer
Nov 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A VallbrachtB Flehmig
Jan 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·V Gauss-Müller, F Deinhardt
Aug 1, 1996·The Journal of General Virology·T PöyryG Stanway
Jan 29, 2002·Nucleic Acids Research·C WitwerP F Stadler
Aug 21, 2002·Journal of Virology·Mauro Costa-MattioliVirginie Ferré
Aug 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guilhermo RezendeCyrille Féray
Jun 17, 2004·The Journal of Clinical Investigation·Steven E Kauder, Vincent R Racaniello
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Jan 18, 2006·Journal of Virology·Krishnamurthy Konduru, Gerardo G Kaplan
Dec 2, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Keiichi FujiwaraOsamu Yokosuka

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Citations

Jan 1, 1997·Annual Review of Microbiology·J R Battista
Jun 3, 2014·International Journal of Molecular Sciences·Feng QiuShengli Bi
Jun 17, 2011·Journal of Medical Virology·Young Kyung YoonMin Ja Kim
Dec 10, 2013·Virology·Soledad García-NuñezElisa Carrillo
Nov 4, 2016·Scientific Reports·Francisco J Pérez-RodríguezRosa M Pintó
Aug 29, 2019·Reviews in Medical Virology·Cédric HartardEvelyne Schvoerer
Jul 14, 2011·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)
Aug 28, 2018·Frontiers in Genetics·Akitoshi SadahiroToshinobu Fujiwara
Apr 13, 2021·Virulence·Rosa M PintóAlbert Bosch

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