Nucleotides induce IL-6 release from human airway epithelia via P2Y2 and p38 MAPK-dependent pathways

American Journal of Physiology. Lung Cellular and Molecular Physiology
Christelle D DouilletPreston B Rich

Abstract

Extracellular nucleotides can mediate a variety of cellular functions via interactions with purinergic receptors. We previously showed that mechanical ventilation (MV) induces airway IL-6 and ATP release, modifies luminal nucleotide composition, and alters lung purinoceptor expression. Here we hypothesize that extracellular nucleotides induce secretion of IL-6 by small airway epithelial cells (SAEC). Human SAEC were stimulated with nucleotides in the presence or absence of inhibitors. Supernatants were analyzed for IL-6 and lysates for p38 MAPK activity by ELISA. RNA was analyzed by real-time RT-PCR. Rats (n=51) were randomized to groups as follows: control, small-volume MV, large-volume MV, large-volume MV-intratracheal apyrase, or small-volume MV-intratracheal adenosine 5'-O-(3-thiotriphosphate) (ATPgammaS). After 1 h of MV, bronchoalveolar lavage fluid was analyzed for ATP and IL-6 by luminometry and ELISA. ATP and ATPgammaS increased SAEC IL-6 secretion in a time- and dose-dependent manner, an effect inhibited by apyrase. Agonists were ranked in the following order: ATPgammaS>ATP=UTP>ADP=adenosine>2-methylthio-ADP=control. SB-203580, but not U-0126 or JNK1 inhibitor, decreased nucleotide effects. Additionally, nucleotides i...Continue Reading

References

Mar 1, 1995·American Journal of Respiratory and Critical Care Medicine·M R KnowlesR C Boucher
Jun 1, 1996·American Journal of Respiratory and Critical Care Medicine·W D BennettM R Knowles
Mar 1, 1997·The Journal of Clinical Investigation·L TremblayA S Slutsky
Oct 6, 1997·The European Respiratory Journal·L D MartinK B Adler
Dec 31, 1997·The American Journal of Physiology·S S GreenbergJ Ouyang
Sep 5, 1998·The American Journal of Physiology·C HierholzerD J Tweardy
Dec 9, 1998·The American Journal of Physiology·E Schulze-LohoffR B Sterzel
Jan 16, 1999·Biochimica Et Biophysica Acta·S PedersenE K Hoffmann
Oct 19, 1999·The Journal of Clinical Investigation·J A EliasR J Homer
Nov 11, 1999·Pflügers Archiv : European journal of physiology·S K InglisR E Olver
Dec 22, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S HashimotoT Horie
Mar 29, 2000·Biochemical Pharmacology·M Williams, M F Jarvis
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S D GriegoS B Dillon
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D FerrariF Di Virgilio
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·I von Kügelgen, A Wetter
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S TavakoliJ H Shelhamer
Apr 24, 2001·American Journal of Respiratory and Critical Care Medicine·J D RicardG Saumon
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J L ChunnM R Blackburn
Dec 14, 2001·Biochemical and Biophysical Research Communications·M RomanelloP D'Andrea

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Citations

Nov 21, 2007·Pflügers Archiv : European journal of physiology·C S GeytiH A Praetorius
Jul 19, 2013·Journal of Molecular Neuroscience : MN·Maosheng Xia, Yue Zhu
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Fiona M SansomElizabeth L Hartland
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin YuJonathan A Dranoff
Mar 2, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michel FaustherMaryse Picher
Jan 1, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Andreas SchwingshacklChristopher M Waters
Dec 9, 2008·American Journal of Respiratory Cell and Molecular Biology·Kyoung Seob SongJoo-Heon Yoon
Mar 7, 2009·American Journal of Respiratory Cell and Molecular Biology·Emilie ThéâtreCécile Oury
Oct 9, 2012·Journal of Dental Research·J C Lim, C H Mitchell
Jun 3, 2009·Critical Care Medicine·Chin-Ming ChenArthur S Slutsky
Jul 17, 2016·Purinergic Signalling·Juliana I SesmaEduardo R Lazarowski
Aug 14, 2012·Pharmacological Reviews·Geoffrey BurnstockJean-Pierre Timmermans
Mar 30, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhi ZhangMin Qian
Dec 16, 2016·Journal of Gastroenterology and Hepatology·Liping WuHiroto Miwa
Apr 8, 2020·Toxins·Rudolf LucasJürg Hamacher
Oct 10, 2019·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ju Jing TanRyszard Grygorczyk
Aug 15, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Timothy S KountzMurali Prakriya

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