Nucleotides protect rat brain astrocytes against hydrogen peroxide toxicity and induce antioxidant defense via P2Y receptors

Neurochemistry International
Daniel Förster, Georg Reiser

Abstract

Consequences of neurodegenerative diseases or stroke also depend on astroglial survival during oxidative stress. P2Y receptors that are widely distributed in the central nervous system are suggested to be involved in cytoprotection. However, knowledge about the efficacy of protection by P2Y receptors and their involvement in antioxidant protective pathways is scarce. Here, we investigate the viability and reactive oxygen species (ROS) production after exposure of rat astrocytes to hydrogen peroxide. We determined the influence of treatment with the P2Y1 receptor-specific agonist 2-methyl-thio-ADP (2MeSADP) and the broad range P2Y receptor agonist adenosine 5'-(3-thiotriphosphate) (ATPγS). Preincubation (24-h before hydrogen peroxide application) and incubation with ATPγS and 2MeSADP protected astrocytes. The ROS production in hydrogen peroxide-treated astrocytes was reduced by pre- and co-incubation with ATPγS or 2MeSADP. Changes of levels of expression of antioxidant defense systems in astrocytes by treatment with P2Y receptor agonists were analyzed. Incubation with ATPγS and 2MeSADP increased mRNA levels of CAT encoding catalase and SOD2, encoding mitochondrial manganese dependent superoxide dismutase. ATPγS additionally incr...Continue Reading

References

Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·R M LebovitzM M Matzuk
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Nov 5, 2002·The Journal of Biological Chemistry·Silvia CocoClaudia Verderio
Jan 14, 2003·EMBO Reports·Jorge MoscatPaul Rennert
May 20, 2003·Biological Chemistry·Ralf Dringen, Johannes Hirrlinger
Apr 14, 2004·Current Topics in Medicinal Chemistry·Peter Illes, Joaquim A Ribeiro
Dec 2, 2004·Journal of Neuroscience Research·Ralf DringenJohannes Hirrlinger
Nov 1, 2005·Pharmacology & Therapeutics·Ivar von Kügelgen
Jan 18, 2006·The Journal of Investigative Dermatology·Kristina SeiffertRichard D Granstein
Apr 28, 2006·Pflügers Archiv : European journal of physiology·Yuri PankratovR Alan North
May 9, 2006·Antioxidants & Redox Signaling·James MeleArlan Richardson
Jul 11, 2006·Biochimica Et Biophysica Acta·Dmitry B ZorovSteven J Sollott
Aug 29, 2006·Biochemical Pharmacology·Liaman K MamedovaKenneth A Jacobson
Sep 22, 2006·Antioxidants & Redox Signaling·Nathan R BradyRoberta A Gottlieb
May 17, 2007·The Journal of General Physiology·David N Bowser, Baljit S Khakh
Jul 31, 2007·Glia·Luc PellerinPierre J Magistretti
Jul 5, 2008·Purinergic Signalling·Kenneth A JacobsonHyojin Ko
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Sep 18, 2008·Molecular Aspects of Medicine·Henry Jay FormanAlessandra Rinna
Sep 25, 2008·Molecular Aspects of Medicine·Christopher C FranklinTerrance J Kavanagh
Oct 15, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G Burnstock
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Apr 29, 2009·Hypertension·Francesco AddabboMichael S Goligorsky
May 12, 2009·Free Radical Biology & Medicine·Alicia J KowaltowskiAnibal E Vercesi
May 19, 2009·Cardiovascular Research·Dmitry B ZorovSteven J Sollott
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·Michelle L Halls, Dermot M F Cooper
Apr 1, 2011·Journal of Inorganic Biochemistry·Sandra Rachmilovich-CalisDan Meyerstein
May 28, 2011·Free Radical Research·Nilanjan GhoshSubhash C Mandal
Sep 29, 2011·Molecular and Cellular Endocrinology·Alexei VerkhratskyVladimir Parpura
Sep 22, 2012·Biochimica Et Biophysica Acta·Shelly C Lu
Dec 4, 2012·Nutrients·William M JohnsonJohn J Mieyal

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Citations

May 10, 2018·The Journal of Physiology·Jan Marino RamirezIbis M Agosto-Marlin
Aug 6, 2018·Journal of Cellular Physiology·Katarzyna Roszek, Magdalena Wujak
Aug 29, 2019·Molecular Genetics & Genomic Medicine·Shuaiqi SunYing Xia
Jun 28, 2018·Frontiers in Molecular Neuroscience·Yu-Feng Wang, Vladimir Parpura
Feb 19, 2017·Molecular and Cellular Biochemistry·Maria D DănilăAdrian Sturza
Jan 14, 2021·International Journal of Molecular Sciences·Juan Carlos Gil-RedondoEsmerilda G Delicado

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