NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression

Oncogene
Sheng TanTao Zhu

Abstract

Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism and involved in many diseases, including cancer. CFIm25, a subunit of the cleavage factor I encoded by NUDT21, is required for 3'RNA cleavage and polyadenylation. Although it has been recently reported to be involved in glioblastoma tumor suppression, its roles and the underlying functional mechanism remain unclear in other types of cancer. In this study, we characterized NUDT21 in hepatocellular carcinoma (HCC). Reduced expression of NUDT21 was observed in HCC tissue compared to adjacent non-tumorous compartment. HCC patients with lower NUDT21 expression have shorter overall and disease-free survival times than those with higher NUDT21 expression after surgery. Knockdown of NUDT21 promotes HCC cell proliferation, metastasis, and tumorigenesis, whereas forced expression of NUDT21 exhibits the opposite effects. We then performed global APA site profiling analysis in HCC cells and identified considerable number of genes with shortened 3'UTRs upon the modulation of NUDT21 expression. In particular, we further characterized the NUDT21-regulated genes PSMB2 and CXXC5. We found NUDT21 knockdown increases usage of the proximal polyadenylation s...Continue Reading

References

Dec 24, 2003·Molecular Cell·Kirk M Brown, Gregory M Gilmartin
Jun 7, 2005·Genes & Development·Krishnan VenkataramanGregory M Gilmartin
May 1, 2008·Nucleic Acids Research·Molly CosenoSylvie Doublié
Feb 17, 2009·Molecular Cell·Yongsheng ShiJames L Manley
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Qin YangSylvie Doublié
Feb 18, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S KnappskogF Pendino
Sep 20, 2011·Molecular Cell·Dafne Campigli Di GiammartinoJames L Manley
Feb 23, 2012·Lancet·Alejandro FornerJordi Bruix
Aug 10, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Adam R MorrisReuven Agami
Jun 19, 2013·Nature Reviews. Genetics·Ran ElkonReuven Agami
May 13, 2014·Nature·Chioniso P MasamhaEric J Wagner
Aug 6, 2014·Nature Reviews. Clinical Oncology·Josep M Llovet
Apr 15, 2016·Molecular Cancer Research : MCR·Ayse Elif Erson-Bensan, Tolga Can
May 10, 2016·Nature Reviews. Disease Primers·Josep M LlovetGregory Gores
Nov 4, 2016·Nature Reviews. Molecular Cell Biology·Bin Tian, James L Manley

❮ Previous
Next ❯

Citations

Nov 28, 2018·Journal of Cellular and Molecular Medicine·Xiangyang XiongXiaohua Yan
Mar 19, 2019·Journal of Cellular and Molecular Medicine·Xiao LangWeiwei Cheng
Jul 10, 2020·Technology in Cancer Research & Treatment·Xiaojie SunXiangwei Meng
Aug 6, 2020·OncoTargets and Therapy·Xiang GaoYuxing Xu
Apr 30, 2020·Experimental & Molecular Medicine·Hee Doo Yang, Suk Woo Nam
Sep 7, 2019·The Journal of Biological Chemistry·Junsuk KoMichael R Blackburn
Sep 17, 2020·International Journal of Molecular Sciences·Francesca CaponeSusan Costantini
May 21, 2020·Cancer Management and Research·Bi-Jun WangWen-Yong Wu
Oct 11, 2020·Molecular Cancer Research : MCR·Xiaonan WangZhengsheng Wu
Oct 25, 2019·Genes & Diseases·Fuwen YuanQianben Wang
Apr 2, 2020·Biochemical and Biophysical Research Communications·Chuan-Cheng GaoLi-Sheng Wang
Mar 3, 2021·Journal of Experimental & Clinical Cancer Research : CR·Sheng TanXiaodong Zhao
Sep 29, 2021·Wiley Interdisciplinary Reviews. RNA·Neeraja K MohananRakesh S Laishram
Oct 9, 2021·Frontiers in Cell and Developmental Biology·Xiaoyan WuXiaolei Shi
Dec 18, 2021·Science Advances·Aishwarya SubramanianW Brent Derry

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
xenograft

Software Mentioned

CULI
bowite
TargetScan

Related Concepts

Related Feeds

Cancer Stem Cells in Glioblastoma

Glioblastoma is the most common and aggressive type of brain tumor. It contains a population of tumor initiating stem cell-like cells known as cancer stem cells. Investigations are ongoing into these cancer stem cells found in these solid tumors which are highly resistance to treatment. Here is the latest research on cancer stem cells in glioblastoma.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.