Nukacin 3299, a lantibiotic produced by Staphylococcus simulans 3299 identical to nukacin ISK-1

Veterinary Microbiology
Hilana CeottoMaria do Carmo de Freire Bastos

Abstract

Nukacin 3299 (formerly designated simulancin 3299), produced by a Staphylococcus simulans strain involved in bovine mastitis in Brazil, is the first peptide bacteriocin described in this staphylococcal species. With the intent to elucidate some aspects of its biology, nukacin 3299 was purified and characterized. The mass of the purified bacteriocin was shown to be 2957.3 Da, and the peptide N-terminal amino acids (KKKSGVI) were identified by Edman degradation. The nukacin 3299 structural gene, nukA, was detected by PCR and DNA sequencing, showing that this bacteriocin is identical to nukacin ISK-1, a 27-amino acid type-A (II) lantibiotic produced by Staphylococcus warneri ISK-1, isolated from a "nukadoko", in Japan. The genes involved in nukacin 3299 biosynthesis are located on plasmid pRJ97 (>27 kb). They have an organization similar to that of the nukacin ISK-1 gene cluster, excepted for the presence of an IS257/431 element (791 bp) present between the orf1 and nukA genes of the nukacin 3299 gene cluster. The presence of this insertion sequence is expected to affect the expression of orf1, whose function is presently unknown. Nukacin 3299 proved to be sensitive to proteolytic enzymes and relatively stable at different tempera...Continue Reading

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Citations

Nov 3, 2015·Research in Microbiology·Marcus Lívio Varella CoelhoMaria do Carmo de Freire Bastos
Nov 20, 2014·Microbiology·Maria do Carmo de Freire BastosOlinda Cabral da Silva Santos
May 29, 2016·International Journal of Food Microbiology·María Sánchez MainarFrédéric Leroy
Jul 2, 2017·Applied and Environmental Microbiology·Domonique A CarsonJeroen De Buck
Feb 10, 2021·Pathogens·Angela FrançaLuís D R Melo
Apr 27, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Felipe Miceli FariasRaquel Regina Bonelli
Jun 3, 2021·Nature Reviews. Microbiology·Simon HeilbronnerAndreas Peschel

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