PMID: 9429079Jan 16, 1998Paper

Number, fixation properties, dye-binding and protease expression of duodenal mast cells: comparisons between healthy subjects and patients with gastritis or Crohn's disease

The Histochemical Journal
W J BeilA F Walls

Abstract

There is an accumulation of evidence to suggest that mast cells may play a key role in gastrointestinal inflammation. We have investigated the numbers and heterogeneity in staining properties of mast cells in biopsies of the duodenum of normal subjects (n = 10), and of normal duodenum from patients with Crohn's disease of the ileum and/or colon (n = 7) or with Helicobacter-associated gastritis of the antrum/corpus (n = 6). In normal donors, two subsets of mast cells, one located in the duodenal mucosa and the other in the submucosa, were clearly distinguished by their morphology and dye-binding properties. Whereas submucosal mast cells stained metachromatically with Toluidine Blue after neutral formalin fixation and emitted a yellow fluorescence after staining with Berberine sulphate, those in the mucosa were invisible using these stains. In patients with gastritis or Crohn's disease, there were marked changes in the numbers of mucosal mast cells compared with control subjects even though the duodenal biopsies were from apparently uninvolved tissue. Gastritis was associated with increased mucosal mast cell numbers (controls: 187 +/- 23 cells mm-2; gastritis: 413 +/- 139 cells mm-2; p = 0.0004), but mean mucosal mast cell counts...Continue Reading

Citations

Dec 6, 2005·Virchows Archiv : an International Journal of Pathology·Francesca MinneiKarel Geboes
Mar 27, 2010·Basic Research in Cardiology·Shizu OyamadaFrank W Sellke
Mar 21, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jens RuschmannGerald Krystal
Feb 5, 2004·World Journal of Gastroenterology : WJG·Shao-Heng He, Hua Xie
Jul 26, 2011·The International Journal of Artificial Organs·Ali GhodsizadArjang Ruhparwar
Jul 19, 2001·Japanese Journal of Pharmacology·D JinM Miyazaki
Sep 20, 2000·The Journal of Experimental Medicine·C T MierkeS C Bischoff
Oct 16, 2004·World Journal of Gastroenterology : WJG·Xiao-Yu ChenWei-Qin Qi
Apr 17, 2013·Biochemistry. Biokhimii︠a︡·T S Zamolodchikova
Sep 24, 2005·International Archives of Allergy and Immunology·Maria-Theresa KrauthPeter Valent
Aug 26, 1998·European Journal of Pharmacology·S He, A F Walls

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