Number of treatment cycles influences development of cytotoxic T cells in metastatic breast cancer patients - a phase I/II study

Immunological Investigations
S E WrightChithraleka Sundaramurthy

Abstract

The influence of the number of apheresis-stimulation-infusion(s) cycles, and the time in culture before the infusion (one vs. two weeks), on the generation of tumor antigen-specific cytotoxic T-lymphocytes (CTL) was investigated in a phase I/II clinical adoptive immunotherapy trial. Two previously treated metastatic breast cancer patients with no evidence of disease, in complete remission (CR), were enrolled. Each apheretic peripheral blood mononuclear cell (PBMC) sample was stimulated twice with MUC-1 before infusion back into the patients. Killer T-cells responses against MUC-1-expressing MCF-7 (CTL), nonspecific natural killer (NK) and lymphokine-activated killer (LAK) target cell lines, as well as, cytokine production were measured before each infusion. Patients received 2 infusions per month for 4 months. There were no tumor recurrences or toxicity. CTL, NK and LAK cells, type 1 cytokine, gamma-interferon (G-INF), and CD4(+) and CD8(+) memory T-lymphocytes were initially generated, produced or induced, respectively, and then declined. The CTL, NK and LAK cells were only induced at the first infusion of the first month. Thus, maintaining PBMC in culture longer than the first infusion was of no benefit with regards to retain...Continue Reading

References

Sep 1, 1991·The American Review of Respiratory Disease·S J GendlerJ Taylor-Papadimitriou
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L BarndO J Finn
May 1, 1988·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S L TopalianP Simon
Feb 12, 1998·The Journal of Clinical Investigation·V KaranikasI F McKenzie
Mar 21, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·N T UenoR E Champlin
Feb 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Malcolm S MitchellJune Kan-Mitchell
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norbert MeidenbauerAndreas Mackensen
Feb 1, 1964·Lancet·J V PULVERTAFT
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juhua ZhouPaul F Robbins
Nov 1, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Andreas MackensenReinhard Andreesen
Jun 21, 2008·The New England Journal of Medicine·Naomi N HunderCassian Yee
Sep 24, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark E DudleySteven A Rosenberg

❮ Previous
Next ❯

Citations

Sep 4, 2012·Immunological Investigations·Kate Rittenhouse-Olson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.