PMID: 9162738Feb 1, 1997Paper

Numerical aberrations of chromosome 7 detected in 15 microns paraffin-embedded tissue sections of primary cutaneous melanomas by fluorescence in situ hybridization and confocal laser scanning microscopy

Journal of Cutaneous Pathology
I D'AlessandroH Höfler

Abstract

Fluorescence in situ hybridization (FISH) to sections of formalin-fixed paraffin-embedded archival tissues allows the detection of gene or chromosome copy number changes in interphase cell nuclei within the histological context and thus may be of particular interest in tumor pathology. In this report, we describe the application of FISH to thick (15 microns) paraffin sections of 7 primary cutaneous malignant melanomas. A chromosome 7-specific centromeric DNA probe was used to detect numerical aberrations of chromosome 7. By optical sectioning using confocal laser scanning microscopy (CLSM) only complete, uncut interphase cell nuclei were scored. The mean percentage (+/- SEM) of melanoma cell nuclei with three hybridization spots was (20.7 +/- 2.8)%; (6.8 +/- 1.0)% of nuclei showed one spot and (5.0 +/- 1.2)% four or more spots. The frequency distribution of spot numbers among melanoma cell nuclei and normal keratinocyte nuclei was significantly different (chi(2) = 176.8, df = 5, p < 0.001). Trisomy 7 was detected in all 7 cases analyzed, mostly associated with monosomy 7 or polysomy 7. The approach used in our study and the data obtained could be useful for further studies designed to investigate a possible involvement of chrom...Continue Reading

References

Mar 15, 1992·Cancer·J W Gray, D Pinkel
Sep 21, 1988·Journal of the National Cancer Institute·J M CowanU Francke

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Citations

Sep 15, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jinglan LiuJean-Pierre de Chadarévian
Feb 28, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Michael BzorekLinda Hansen
Aug 23, 2011·The Breast : Official Journal of the European Society of Mastology·E KouvarasG K Koukoulis
Jan 22, 2011·Journal of Cutaneous Pathology·Bryan GammonPedram Gerami
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sandra RauserAxel Walch
Apr 29, 2006·The Journal of Molecular Diagnostics : JMD·Roland A VenturaReiner Siebert

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