Numerical analysis of Ca2+ depletion in the transverse tubular system of mammalian muscle

Biophysical Journal
O FriedrichR H A Fink

Abstract

Calcium currents were recorded in contracting and actively shortening mammalian muscle fibers. In order to characterize the influence of extracellular calcium concentration changes in the small unstirred lumina of the transverse tubular system (TTS) on the time course of the slow L-type calcium current (I(Ca)), we have combined experimental measurements of I(Ca) with quantitative numerical simulations of Ca2+ depletion. I(Ca) was recorded both in calcium-buffered and unbuffered external solutions using the two-microelectrode voltage clamp technique (2-MVC) on short murine toe muscle fibers. A simulation program based on a distributed TTS model was used to calculate the effect of ion depletion in the TTS. The experimental data obtained in a solution where ion depletion is suppressed by a high amount of a calcium buffering agent were used as input data for the simulation. The simulation output was then compared with experimental data from the same fiber obtained in unbuffered solution. Taking this approach, we could quantitatively show that the calculated Ca2+ depletion in the transverse tubular system of contracting mammalian muscle fibers significantly affects the time-dependent decline of Ca2+ currents. From our findings, we c...Continue Reading

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Citations

Dec 15, 2010·The Journal of General Physiology·Marino DiFrancoJulio L Vergara
Sep 29, 2011·The Journal of General Physiology·Marino DiFranco, Julio L Vergara
May 19, 2007·Theoretical Biology & Medical Modelling·Paul R Shorten, Tanya K Soboleva
Jan 26, 2011·The Journal of Physiology·Marino DiFrancoJulio L Vergara
Jul 2, 2008·The Journal of Physiology·S P Cairns, M I Lindinger
Nov 5, 2003·The Journal of Physiology·O FriedrichR H A Fink
Jun 16, 2007·The Journal of Physiology·Bradley S Launikonis, Eduardo Ríos
Dec 30, 2014·The Journal of Physiology·Marino DiFrancoJulio L Vergara
Sep 6, 2008·The Journal of Physiology·Joshua N Edwards, Bradley S Launikonis
Jul 17, 2007·Biophysical Journal·P R ShortenT K Soboleva
Jun 17, 2009·Biophysical Journal·P R Shorten, J Sneyd
Sep 18, 2007·Progress in Biophysics and Molecular Biology·M PásekC H Orchard
Aug 25, 2019·Journal of Muscle Research and Cell Motility·Mudassir Haider RizviArifa Savanur
Oct 3, 2018·Communications Biology·Xaver KoenigBradley S Launikonis
Jul 1, 2005·The Journal of Nutrition·Oliver FriedrichErnst Hund

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