Numerical-experimental observation of shape bistability of red blood cells flowing in a microchannel

Soft Matter
Achim GuckenbergerStephan Gekle

Abstract

Red blood cells flowing through capillaries assume a wide variety of different shapes owing to their high deformability. Predicting the realized shapes is a complex field as they are determined by the intricate interplay between the flow conditions and the membrane mechanics. In this work we construct the shape phase diagram of a single red blood cell with a physiological viscosity ratio flowing in a microchannel. We use both experimental in vitro measurements as well as 3D numerical simulations to complement the respective other one. Numerically, we have easy control over the initial starting configuration and natural access to the full 3D shape. With this information we obtain the phase diagram as a function of initial position, starting shape and cell velocity. Experimentally, we measure the occurrence frequency of the different shapes as a function of the cell velocity to construct the experimental diagram which is in good agreement with the numerical observations. Two different major shapes are found, namely croissants and slippers. Notably, both shapes show coexistence at low (<1 mm s-1) and high velocities (>3 mm s-1) while in-between only croissants are stable. This pronounced bistability indicates that RBC shapes are n...Continue Reading

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Citations

Sep 28, 2018·Soft Matter·Austin NehringHendrick W de Haan
Jun 23, 2020·Soft Matter·Fabio GugliettaMauro Sbragaglia
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May 29, 2021·Soft Matter·Fabio GugliettaMauro Sbragaglia
Sep 22, 2021·Biophysical Journal·Zhe GouChaouqi Misbah
Dec 14, 2021·Biophysical Journal·Steffen M RecktenwaldChristian Wagner

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Methods Mentioned

BETA
light microscopy

Software Mentioned

MATLAB script
GMRES
MATLAB

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