Numerical methods to determine calcium release flux from calcium transients in muscle cells

Biophysical Journal
J TimmerW Melzer

Abstract

Several methods are currently in use to estimate the rate of depolarization-induced calcium release in muscle cells from measured calcium transients. One approach first characterizes calcium removal of the cell. This is done by determining parameters of a reaction scheme from a fit to the decay of elevated calcium after the depolarizing stimulus. In a second step, the release rate during depolarization is estimated based on the fitted model. Using simulated calcium transients with known underlying release rates, we tested the fidelity of this analysis in determining the time course of calcium release under different conditions. The analysis reproduced in a satisfactory way the characteristics of the input release rate, even when the assumption that release had ended before the start of the fitting interval was severely violated. Equally good reconstructions of the release rate time course could be obtained when the model used for the analysis differed in structure from the one used for simulating the data. We tested the application of a new strategy (multiple shooting) for fitting parameters in nonlinear differential equation systems. This procedure rendered the analysis less sensitive to ill-chosen initial guesses of the param...Continue Reading

References

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Citations

Nov 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zoita AndronacheWerner Melzer
Dec 17, 2003·The Journal of General Physiology·R P Schuhmeier, W Melzer
Oct 29, 2014·The Journal of General Physiology·Peter BraubachWerner Melzer
Feb 26, 2005·The Journal of Physiology·Joana CapoteCarlo Caputo
Feb 1, 2005·Biophysical Journal·Alejandra C VenturaSilvina Ponce Dawson
Feb 14, 2009·The FEBS Journal·Clemens Kreutz, Jens Timmer
Jun 20, 2006·Biophysical Journal·Sandrine PouvreauVincent Jacquemond
Jan 28, 2003·Biophysical Journal·R P SchuhmeierW Melzer
Dec 17, 2017·The Journal of General Physiology·Alberto ZulloWerner Melzer
Aug 6, 2010·American Journal of Physiology. Cell Physiology·Benjamin L ProsserMartin F Schneider

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