Numerical, morphological and phenotypic changes in Langerhans cells in the course of murine graft-versus-host disease

The British Journal of Dermatology
K AsagoeT Akagi

Abstract

In the course of graft-versus-host disease (GVHD) or diseases that histologically mimic GVHD (e.g. toxic epidermal necrolysis, Stevens-Johnson syndrome), it is known that epidermal Langerhans cells (LCs) are depleted from the epidermis. However, the mechanism and significance of LC depletion is not well known. To investigate the numerical, morphological and phenotypic changes in LCs and apoptosis of LCs in the course of GVHD using a non-irradiated mouse GVHD model. BALB/c nu/nu mice and C57BL/6 mice were used as recipients and donors, respectively. Recipient mice were injected with T-cell-enriched donor spleen cells. Skin samples were harvested at various times after the inoculation. The numerical and morphological changes were examined by an immunofluorescence study of epidermal sheets. Apoptosis was studied by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labelling method and flow cytometric analysis using annexin V. Phenotypic change was studied by flow cytometric analysis of epidermal cell suspensions. The mixed epidermal cell lymphocyte reaction (MELR) was performed to examine functional changes in the epidermal cells. Five days after inoculation, a graft-versus-host reaction occurre...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H Enk, S I Katz
Mar 7, 1991·The New England Journal of Medicine·J L Ferrara, H J Deeg
Nov 1, 1990·The Journal of Experimental Medicine·C P LarsenJ M Austyn
Dec 1, 1990·Journal of the American Academy of Dermatology·J C RoujeauJ C Guillaume
Aug 1, 1987·The American Journal of Dermatopathology·S M Breathnach, S I Katz
Aug 1, 1988·The Journal of Investigative Dermatology·B Volc-PlatzerK Wolff
Mar 1, 1985·The Journal of Experimental Medicine·G Schuler, R M Steinman
Nov 1, 1983·Transplantation·A J Suitters, I A Lampert
Nov 1, 1983·The Journal of Investigative Dermatology·R J MargolisN A Soter
Nov 10, 1994·The New England Journal of Medicine·J C Roujeau, R S Stern
Jan 1, 1994·The Journal of Dermatology·J OsawaH Nakajima
Apr 1, 1996·The British Journal of Dermatology·C PaulJ C Roujeau
Sep 1, 1996·The Journal of Investigative Dermatology·A C GilliamG F Murphy
Feb 1, 1997·Transplantation Proceedings·T WakabayashiH Uchida
Jun 1, 1997·Immunological Reviews·B R BlazarD A Vallera
Mar 31, 1998·Journal of the American Academy of Dermatology·M L Johnson, E R Farmer
Jun 2, 1998·Archives of Dermatology·S Aractingi, O Chosidow
Jun 3, 1999·The New England Journal of Medicine·E C GuinanJ G Gribben

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Citations

Oct 1, 2005·Leukemia & Lymphoma·D Nachbaur, B Kircher
Feb 24, 2011·The Journal of Dermatology·Hiroaki Azukizawa
Feb 23, 2008·Annals of Medicine·Ribhi HazinArash Kimyai-Asadi

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