Mar 30, 2014

Development and Testing of New Genetic Markers for the Detection of Invasive Bighead and Silver Carp (Hypophthalmichthys nobilis and H. molitrix) DNA in environmental water samples from North America

BioRxiv : the Preprint Server for Biology
Richard F. LanceKelly Baerwaldt


Invasive Asian bighead and silver carp ( Hypophthalmichthys nobilis and H. molitrix ) pose a substantial threat to North American waterways. Recently, environmental DNA (eDNA), the use of species-specific genetic assays to detect the DNA of a particular species in a water sample, has gained recognition as a tool for tracking the invasion front of these species toward the Great Lakes. The goal of this study was to develop new species-specific conventional PCR (cPCR) and quantitative (qPCR) markers for detection of these species in North American waterways. We first generated complete mitochondrial genome sequences from 33 bighead and 29 silver carp individuals collected throughout their introduced range. These sequences were aligned with other common and closely related species to identify potential eDNA markers. We then field-tested these genetic markers for species-specificity and sensitivity in environmental samples. Newly developed markers performed well in field trials, had low false positive rates and had comparable sensitivity compared to current markers. The new markers developed in this study greatly expand the number of species-specific genetic markers available to track the invasion front of bighead and silver carp, a...Continue Reading

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