NUPR1 acts as a pro-survival factor in human bone marrow-derived mesenchymal stem cells and is induced by the hypoxia mimetic reagent deferoxamine

Journal of Clinical Biochemistry and Nutrition
Kazuhito MatsunagaIsao Sakaida

Abstract

Differences in the culturing conditions of mesenchymal stem cells used in regenerative medicine may affect their differentiation ability, genome instability, and therapeutic effects. In particular, bone marrow-derived mesenchymal stem cells cultured under hypoxia are known to proliferate while maintaining an undifferentiated state and the use of deferoxamine, a hypoxia mimetic reagent, has proven to be a suitable strategy to maintain the cells under hypoxic metabolic state. Here, the deferoxamine effects were investigated in mesenchymal stem cells to gain insights into the mechanisms regulating stem cell survival. A 12-h deferoxamine treatment reduced proliferation, oxygen consumption, mitochondrial activity, and ATP production. Microarray analysis revealed that deferoxamine enhanced the transcription of genes involved in glycolysis and the HIF1α pathway. Among the earliest changes, transcriptional variations were observed in HIF1α, NUPR1, and EGLN, in line with previous reports showing that short deferoxamine treatments induce substantial changes in mesenchymal stem cells glycolysis pathway. NUPR1, which is induced by stress and involved in autophagy-mediated survival, was upregulated by deferoxamine in a concentration-depende...Continue Reading

References

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Citations

Apr 2, 2020·Journal of Clinical Biochemistry and Nutrition·Lailan Safina NasutionMohamad Sadikin
Jul 31, 2020·Cell Stress & Chaperones·Nasrin KhoshlahniMohammad Mohammadzadeh-Vardin
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Lei JiangZhensheng Qin

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Methods Mentioned

BETA
flow cytometry
Assay
Chip
environmental stresses

Software Mentioned

IPA
Ingenuity Pathways Analysis ( IPA

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