PMID: 7539140May 23, 1995Paper

NusA interferes with interactions between the nascent RNA and the C-terminal domain of the alpha subunit of RNA polymerase in Escherichia coli transcription complexes

Proceedings of the National Academy of Sciences of the United States of America
K Liu, M M Hanna

Abstract

The effects of NusA on the RNA polymerase contacts made by nucleotides at internal positions in the nascent RNA in Escherichia coli transcription complexes were analyzed by using the photocrosslinking nucleotide analog 5-[(4-azidophenacyl) thio]-UMP. It was placed at nucleotides between +6 and +15 in RNA transcribed from the phage lambda PR' promoter. Crosslinks of analog in these positions in RNAs which contained either 15, 28, 29, or 49 nt were examined. Contacts between the nascent RNA and proteins in the transcription complex were analyzed as the RNA was elongated, by placing the crosslinker nearest the 5' end of the RNA 10, 23, 24, or 44 nt away from the 3' end. The beta or beta' subunit of polymerase, and NusA when added, were contacted by RNA from 15 to 49 nt long. When the upstream crosslinker was 24 nt from the 3" end of the RNA (29-nt RNA), alpha was also contacted in the absence of NusA. The addition of NusA prevented RNA crosslinking to alpha. When the crosslinker was 44 nt from the 3' end (49-nt RNA), alpha crosslinks were still observed, but crosslinks to beta or beta' and NusA were greatly diminished. RNA crosslinking to alpha, and loss of this crosslink when NusA was added, was observed in the presence of NusB, ...Continue Reading

References

Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·D A ErieP H von Hippel
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Citations

Jul 30, 2002·The EMBO Journal·Nicolas BischlerPatrick Schultz
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R H Ebright
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·J GreenblattL E Kay
Aug 30, 2008·Annual Review of Microbiology·Jeffrey W RobertsJoshua J Filter
Mar 31, 2016·Nucleic Acids Research·Christopher D WellsAnn Hochschild
Aug 6, 2003·Journal of Molecular Biology·Claudio O GualerziCynthia L Pon
May 18, 1999·Journal of Molecular Biology·E Nudler
Nov 5, 1999·Journal of Molecular Biology·S Busby, R H Ebright

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