Nutrient broth/PEG200/TritonX114/Tween80/Chloroform microemulsion as a reservoir of solubilized sitosterol for biotransformation to androstenedione.

Journal of Industrial Microbiology & Biotechnology
Alok Malaviya, James Gomes

Abstract

Microemulsions (ME) can act as a reservoir of solubilized hydrophobic substrates. The biotransformation of hydrophobic sitosterol to androstenedione (AD) with MEs prepared from nutrient broth and PEG 200 (1:1) as aqueous phase, 40 g/l sitosterol dissolved in chloroform as organic phase, Triton X114 and Tween 80 (1:1) as surfactant phase, was investigated. The phase behavior of this system was studied for ten different ratios(w/w), 10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9 and 0:10 of the organic phase and surfactant at 30 degrees C. A pseudoternary phase diagram was constructed to demarcate the region giving stable MEs. The maximum solubility of sitosterol in ME medium was observed to be 8 g/l, which is 3 orders of magnitude higher than the reported sitosterol solubility of 2-4 mg/l in aqueous medium. The ME medium was used for biotransformation studies and a comparative result has been reported. Transmission electron microscopy of cells grown in ME having oil, surfactant and aqueous phase in the ratio of 6:14:80 showed a weakened cell wall structure that permitted production of 465.86 mg/l AD.

References

Jun 23, 2000·Enzyme and Microbial Technology·S PrichanontD C Stuckey
Apr 9, 2008·Journal of Industrial Microbiology & Biotechnology·Zhilong WangDaijie Chen
Jul 1, 1987·Biotechnology and Bioengineering·C LaaneC Veeger
Dec 5, 1991·Biotechnology and Bioengineering·A J SmoldersJ M Cabral

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Citations

May 9, 2012·Applied Microbiology and Biotechnology·Marina V Donova, Olga V Egorova
Feb 4, 2009·Applied Biochemistry and Biotechnology·Alok Malaviya, James Gomes
May 5, 2010·Enzyme and Microbial Technology·Zhilong Wang, Zewen Dai
Jul 23, 2016·Drug Testing and Analysis·A I DecloedtL Vanhaecke
Dec 3, 2017·Applied Biochemistry and Biotechnology·Rodrigo A MancillaAlejandro Amoroso
Mar 11, 2008·Bioresource Technology·Alok Malaviya, James Gomes

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