Nutrient leaching and end product accumulation in plastic composite supports for L-(+)-lactic Acid biofilm fermentation.

Applied and Environmental Microbiology
K G HoA Demirci

Abstract

Investigations on the leachate bioavailability, leaching rate, and lactic acid accumulation properties of plastic composite supports (PCS) were essential for large-scale or long-term lactic acid fermentation. Leachates from PCS and polypropylene discs (controls) were analyzed by the micro-Kjeldahl method; by absorbances at 260, 275, and 280 nm; and by bioassays with Lactobacillus casei subsp. rhamnosus (ATCC 11443). The amount of leached nitrogen in a 20-ml initial soaking solution had a high correlation with the soaking solution's cell density (r = 0.87) and absorbance at 260 nm (r = 0.95). Leaching rates of various PCS were evaluated by 20 20-ml simulated repeated-batch fermentations (RBF). PCS with only yeast extract as the minor agricultural ingredient had a high leaching rate and leached out 51 to 60% of the total nitrogen during the first RBF. PCS blended with dried bovine albumin, dried bovine erythrocytes, and/or soybean flour had slowed nutrient leaching (20 to 30% of the initial leached nitrogen). Hence, they could still maintain 1 g of lactic acid per liter and measurable cell density (absorbance at 620 nm, 0.4 to 0.6) at the 20th 20-ml RBF. Lactic acid accumulation properties of PCS were evaluated by soaking the sup...Continue Reading

References

Apr 1, 1989·Applied and Environmental Microbiology·J S Dickson, M Koohmaraie
Mar 1, 1985·Applied and Environmental Microbiology·A L Pometto, D L Crawford
Aug 1, 1986·Applied and Environmental Microbiology·A L Pometto, D L Crawford
Jan 1, 1993·Applied and Environmental Microbiology·A DemirciK E Johnson

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Citations

Feb 18, 2011·Applied Microbiology and Biotechnology·Kuan-Chen ChengJeffrey M Catchmark
May 10, 2013·Applied Microbiology and Biotechnology·Duygu Ercan, Ali Demirci
May 31, 2006·Applied Microbiology and Biotechnology·Thunyarat Pongtharangkul, Ali Demirci
May 4, 2010·Applied Microbiology and Biotechnology·Kuan-Chen ChengJeffrey M Catchmark
Sep 11, 2008·Biotechnology and Bioengineering·Nagapadma JastiJ Hans van Leeuwen
Mar 13, 2010·Journal of Industrial Microbiology & Biotechnology·Kuan-Chen ChengJeffrey M Catchmark
Aug 8, 2013·Critical Reviews in Biotechnology·Duygu Ercan, Ali Demirci
Dec 18, 2015·Journal of the Science of Food and Agriculture·Chi-Te LiuKuan-Chen Cheng
Jul 28, 2009·Journal of Biological Engineering·Kuan-Chen ChengAli Demirci

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