PMID: 8600464Mar 1, 1996Paper

Nutritional and growth control of ribosomal protein mRNA and rRNA in Neurospora crassa

Nucleic Acids Research
T P Cujec, B M Tyler

Abstract

The effects of changing growth rates on the levels of 40S pre-rRNA and two r-protein mRNAs were examined to gain insight into the coordinate transcriptional regulation of ribosomal genes in the ascomycete fungus Neurospora crassa. Growth rates were varied either by altering carbon nutritional conditions, or by subjecting the isolates to inositol-limiting conditions. During carbon up- or down-shifts, r-protein mRNA levels were stoichiometrically coordinated. Changes in 40S pre-rRNA levels paralleled those of the r-protein mRNAs but in a non-stoichiometric manner. Comparison of crp-2 mRNA levels with those of a crp-2::qa-2 fusion gene indicated no major effect from changes in crp-2 mRNA stability. Crp-2 promoter mutagenesis experiments revealed that two elements of the crp-2 promoter, -95 to -83 bp (Dde box) and -74 to -66 bp (CG repeat) important for transcription under constant growth conditions, are also critical for transcriptional regulation by a carbon source. Ribosomal protein mRNA and rRNA levels were unaffected by changes in growth rates when the cultures were grown under inositol-limiting conditions, suggesting that, under these conditions, transcription of the ribosomal genes in N.crassa was regulated independently of ...Continue Reading

References

May 1, 1976·Experimental Cell Research·E SturaniF A Alberghina
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M E CaseN H Giles
Sep 28, 1978·Nature·J R Warner, C Gorenstein
May 12, 1991·Molecular and Cellular Biochemistry·W H Mager, R J Planta
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N Hariharan, R P Perry
May 5, 1989·Journal of Molecular Biology·R F GeeverN H Giles
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M D DabevaJ R Warner
Aug 24, 1973·Biochimica Et Biophysica Acta·A Grigorescu, C A Villee
Jan 1, 1984·Annual Review of Biochemistry·M NomuraG Baughman
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·V B PatelN H Giles
May 1, 1993·Molecular & General Genetics : MGG·L S KraakmanR J Planta
Feb 1, 1993·Molecular and Cellular Biology·B E MorrowJ R Warner

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